OBJECTIVE: To study the quality standard of hospital preparation Sanwei radix isatidis granules

so as to improve the quality of the preparation. METHODS: Thin Layer chromatography（ TLC） was used for the qualitative identification of Sanwei radix isatidis granules. Ultraviolet and visible spectrophotometry（ UV-Vis） was used to determine the content of polysaccharides

which was one of the total active ingredients of the preparation. High performance liquid chromatography（ HPLC） was used to determine the content of the active ingredient（ R

S）-goitrin in this preparation. RESULTS: According to the qualitative identification by TLC method

radix isatidis and screwtree root in Sanwei isatidis radix isatidis granules showed the same spots in the same position with the corresponding control materials and control substances

and the spots were negative without interference. According to UV-Vis

the content of polysaccharide was 6. 31% and RSD was 0. 67%. The content of（ R

S）-goitrin in the preparation was 0. 035 5 mg/g and RSD was 5. 98% by HPLC method. CONCLUSIONS: Compared with the original quality standard of Sanwei radix isatidis granules

TLC

UV-Vis and HPLC methods are enrolled into the study

which are simple

rapid

stable and reliable

and can be incorporated into the quality standard of hospital preparation Sanwei radix isatidis granules to provide basis for controlling the quality of the preparation.