OBJECTIVE: To explore the possible mechanism of drug pair of cremastrae pseudobulbus pleiones pseudobulbus and gleditsiae spina in treatment of lung cancer based on network pharmacology. METHODS: The active ingredients and targets of drug pair of cremastrae pseudobulbus pleiones pseudobulbus and gleditsiae spina were inquired through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform（ TCMSP）database and then the targets were coverted into gene ID through Uniprot database. The lung cancer related targets were inquired from online mendelian inheritance in man and Genecards database. Venn Diagram was drawn to obtain the intersection of targets and disease targets by Draw Venn Diagram

and String data and Metascape online database were used for enrichment analysis. The results were visualized by Cytoscape 3. 7. 2

showing the screened core targets and protein-protein interaction networks. RESULTS: A total 10 active ingredients and 202 potential targets were obtained. There were 141 possible targets for lung cancer

and 20 core targets were screened. After gene ontology functional enrichment analysis

851 biological processes were involved. After enrichment analysis of the Kyoto Gene and Genome Encyclopedia pathways

110 signaling pathways were involved

among which the IL-17 pathway

MAPK signaling pathway and VEGF pathway were the main pathways that were frequently explored in the development of lung cancer. CONCLUSIONS: The potential mechanism of drug pair of cremastrae pseudobulbus pleiones pseudobulbus and gleditsiae spina in intervention for lung cancer treatment through multi-target and multi-pathway may be related to the inhibition of tumor division and growth

inhibition of angiogenesis near the tumor and control of tumor metastasis.