OBJECTIVE: To measure the contents of isoflavoues aglycone

stibene glucoside

emodin and physcion in raw polygonum multiflorum

steamed polygonum multiflorum and polygonum multiflorum made from black bean juice

so as to provide reference for the formulation of quality control indicators. METHODS: Take polygonum multiflorum made from black bean juice

steamed polygonum multiflorum

and raw polygonum multiflorum powder to make test solution respectively

and take isoflavoues aglycone

black bean

stibene glucoside

emodin and physcion to make reference solution respectively. Thin layer chromatography was adopted to conduct qualitative analysis on test solution and reference solution

and high performance liquid chromatography was adopted to conduct quantitative analysis on test solution and control solution

tests of repeatability

stability

precision and adding sample recovery were preformed. RESULTS: In the chromatography of polygonum multiflorum solution made from black bean juice

fluorescent spots with same color were displayed on the corresponding positions of black bean and isoflavoues aglycone reference substance

while there were no fluorescent spots with same color displayed on the chromatography of raw polygonum multiflorum and steamed polygonum multiflorum. When the sample size of isoflavoues aglycone was in the range of 1. 479-18. 782 μg

it had a linear relationship with the integrated value of corresponding chromatographic peak area. The content of isoflavoues aglycone in polygonum multiflorum made from black bean juice was higher than that in raw polygonum multiflorum and steamed polygonum multiflorum

while the contents of stibene glucoside

emodin and physcion were lower than those in raw polygonum multiflorum and steamed polygonum multiflorum; no isoflavoues aglycone was detected in the test solution of raw polygonum multiflorum and steamed polygonum multiflorum.CONCLUSIONS: Then contents of active ingredient in prepared polygonum multiflorum have changed

the content determination of isoflavoues aglycone can be used as a detection indicator for the identification and quality control of polygonum multiflorum made from black soybean juice and other processing techniques.