OBJECTIVE: To investigate the molecular mechanism of Tanyu Tongzhi formula in the treatment of myocardial ischemia reperfusion injury（ MIRI） based on integrative pharmacology-based research platform of traditional Chinese medicine V2.0（ TCMIP V2.0）.METHODS: TCMIP V2.0 was used to collect drug components

targets and MIRI disease targets of Tanyu Tongzhi formula

and drug-disease target interaction network was established.Common targets of drug and disease were screened

and biological process of the common targets was analyzed.Eventually

multidimensional network analysis of "component-target-pathway-pharmacological action " was established and the core components in the network were analyzed.RESULTS: A total of 157 pharmaceutical chemical components were collected

including pinellia ternata

red peony root

Ligusticum wallichii and licorice

and 272 corresponding targets were identified.A total of 290 targets of MIRI were obtained.Through "Traditional Chinese Medicine Association Network Mining"

the first 100 targets in the core targets were analyzed

and 31 targets for drugs and diseases were found.Common targets mainly participated into the positive regulation of angiogenesis

angiogenesis

positive regulation of autophagy

inflammatory response and negative regulation of apoptosis.Kyoto Encyclopedia of Genes and Genome Encyclopedia pathway enrichment analysis showed that the common targets were mainly enriched in FoxO

HIF-1

TNF

PI3 K-Akt and VEGF signaling pathways.Further multidimensional network analysis showed that 46 components in Tanyu Tongzhi formula affected the above 5 signaling pathways by acting with 19 common targets.Quercetin

kaempferol

naringenin and baicalein participated into the occurrence and development of MIRI through 5 signaling pathways by intervention of AKT.CONCLUSIONS: Quercetin

kaempferol

naringenin and baicalein in Tanyu Tongzhi formula can inhibit MIRI by regulating inflammatory response

autophagy and apoptosis

oxidative stress and angiogenesis.