OBJECTIVE: To probe into the effects of Cinnamomum cassia Presl combined with "Phellodendri Chinensis-Anemarrhenae Rhizoma"on inflammatory factors in kidney and serum samples of rats with pyelonephritis

and to explore the molecular mechanism of Cinnamomum cassia Presl in the treatment of pyelonephritis.METHODS:Rat model of pyelonephritis was established

the control group

model group

positive drug group（ levofloxacin hydrochloride）

Zishen Tongguan prescription group（ Cinnamomum cassia Presl combined with "Phellodendri Chinensis-Anemarrhenae Rhizoma"） and drug combination group（ "Phellodendri Chinensis-Anemarrhenae Rhizoma"）were compared.Serum concentrations of inflammatory factors [interleukin（ IL） 1α

IL-1β

tumor necrosis factor α（ TNF-α）

IL-6

monocyte chemotaxis protein 1（ MCP-1）

CXCL2] in each group were determined by ELISA for pharmacodynamic study.RESULTS:（ 1） The disease condition of rats in the positive drug group

Zishen Tongguan prescription group and drug combination group was improved.Through the comparison of pathological sections

the improvement of rats in the Zishen Tongguan prescription group and the positive drug group was significantly better than that of the"Phellodendri Chinensis-Anemarrhenae Rhizoma"drug combination group.（ 2） The levels of IL-1α

IL-1β

TNF-α

IL-6

MCP-1 and CXCL2 in the model group were higher than those in the control group

and there were statistically significant differences in the levels of IL-1α and CXCL2 in serum of the model group and the control group（ P<0.05）.（ 3） Compared with model group

the levels of inflammatory factors in the positive drug group

Zishen Tongfang prescription group and drug combination group decreased; compared with the model group

there were significant differences in levels of IL-1α in the Zisheng Tongfang prescription group and levels of TNF-α in the positive drug group（ P < 0.05）.CONCLUSIONS: "Phellodendri Chinensis-Anemarrhenae Rhizoma " combined with Cinnamomum cassia Presl can promote the active components of Phellodendri Chinensis and Anemarrhenae Rhizoma to down-regulate the expression of inflammatory factors such as IL-1α

IL-1β

TNF-α

IL-6

MCP-1 and CXCL2

affect the immune response of downstream inflammatory factors

and then play an role in anti-inflammatory and protective effect on the kidney and other urinary systems.