OBJECTIVE: To identify the components（ groups） and targets of coptidis rhizoma and euodiae fructus in the intervention of depression

and explore the mechanism of coptidis rhizoma and euodiae fructus in the treatment of depression. METHODS: The potential active components of coptidis rhizoma and euodiae fructus were screened out from traditional Chinese medicine systems pharmacology database and analysis platform. The target of active components was selected in Swiss Target Prediction database. With the help of the Gene Card database

relevant targets for depression diseases were predicted and extracted. Cytoscape software was used to construct "component-target-disease"network

and STRING database platform was used to construct the protein interaction network. Gene Ontology（ GO） function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes（ KEGG） pathway enrichment analysis were performed by using David platform and R language. RESULTS: A total of 41 potential active components of coptidis rhizoma and euodiae fructus were extrected

including 11 cases of coptidis rhizoma

27 cases ofeuodiae fructus

and3 cases of coptidis rhizoma and euodiae fructus. Through the analysis of "component-target-disease "network node

10 active components of coptidis rhizoma and euodiae fructus were further obtained

which mainly included berberine

β-sitosterol

euodiae fructus rutaecarpine and palmatine. By acting on targets of PTGS2

ACHE

MAOA

AR

BCHE and DRD2

coptidis rhizoma and euodiae fructus could regulate the signal transduction of serotonergic synaptic pathway

neuroactive ligand-receptor interaction pathway

calcium signaling pathway

c AMP receptor pathway and c GMP-PKG pathway in the intervention of depression. CONCLUSIONS: This study preliminarily clarifies the material basis and mechanism of coptidis rhizoma and euodiae fructus in the intervention of depression

and reveals the integrity and systematicness of coptidis rhizoma and euodiae fructus in the treatment of depression to a certain extent.