OBJECTIVE:To probe into the inhibition of Fuzheng Qudu prescription on the human breast cancer MCF-7 cell proliferation and its effects on PI3 K/Akt signaling pathway and the expression of vascular endothelial growth factor-C（VEGF-C） and vascular endothelial growth factor receptor-3（VEGFR-3）.METHODS:The human breast cancer cell strain MCF-7 was cultured in the incubator of completely medium at 37 ℃

5% CO

2

fully saturated humidity to a logarithmic growth cycle

inoculated in a 96-well culture plate

added with concentration gradients（5

10 and 20 μmol/L） of Fuzheng Qudu prescription medium

set normal cultured cells as contrast;after 24 hours of incubation

the thiazole blue method was used to detect cell proliferation and calculate the cell proliferation inhibition rate;the reverse transcription polymerase chain reaction method was used to detect the mRNA levels of VECJF-C and VEGFR-3;Western Blot was used to detect the expression levels of PI3 K and AKT protein.RESULTS:The inhibition rate of cell proliferation in the low

medium and high dose groups of Fuzheng Qudu prescription was significantly higher than that of the control group

with statistically significant difference（P

<

0.05）

which suggested that Fuzheng Qudu prescription had a good inhibitory effect on breast cancer cell proliferation

and as the dose increase

the inhibitory effect more obvious:the mRNA levels of VFGF-C and VFGFR-3 in tne low

medium dose groups of the Fuzheng Qudu prescription were significantly lower than those of the control group

with statistically significant differences（P

<

0.05）

which suggested that Fuzheng Quclu prescription can down-regulate the VEGF-C and VEGFR-3 genes level

and as the dose increase

the decrease became greater;the expression levels of PI3 K and Akt protein in the low

medium and high-dose groups of Fuzheng Qudu prescription were significantly lower than those of the control group

with statistically significant differences（P

<

0.05）

which presented a dose-dependent

and the larger the dose

the more obvious the reduction.CONCLUSIONS:Fuzheng Qudu prescription can inhibit the cell proliferation of breast cancer MCF-7

its mechanism may be related to blocking PI3 K/Akt signaling pathway and down-regulating the expression of VEGF-C and VEGFR-3 genes.