高效液相色谱法测定大鼠血浆中倍半萜内酯类化合物苍耳亭的浓度

曹秀; 韩静文; 吴妍; 李会婷; 马旖旎; 陈卫东; 史天陆

doi:10.14009/j.issn.1672-2124.2020.12.003

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高效液相色谱法测定大鼠血浆中倍半萜内酯类化合物苍耳亭的浓度

论著 | 更新时间：2022-10-08

- 高效液相色谱法测定大鼠血浆中倍半萜内酯类化合物苍耳亭的浓度
- Determination of Concentration of Xanthine in Rat Plasma by High Performance Liquid Chromatography
- 中国医院用药评价与分析 2020年20卷第12期页码：1417-1420
- 作者机构：
  
  1. 安徽中医药大学药学院
  2. 中国科学技术大学附属第一医院(安徽省立医院)药剂科
- 作者简介：
- 基金信息：
  
  安徽省自然科学基金面上项目（No.1708085MH225）
- DOI：10.14009/j.issn.1672-2124.2020.12.003
  中图分类号： R285.5
- 纸质出版日期：2020，
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[1]曹秀,韩静文,吴妍,李会婷,马旖旎,陈卫东,史天陆.高效液相色谱法测定大鼠血浆中倍半萜内酯类化合物苍耳亭的浓度[J].中国医院用药评价与分析,2020,20(12):1417-1420.

CAO Xiu, HAN Jingwen, WU Yan, et al. Determination of Concentration of Xanthine in Rat Plasma by High Performance Liquid Chromatography[J]. 2020,20(12):1417-1420.
[1]曹秀,韩静文,吴妍,李会婷,马旖旎,陈卫东,史天陆.高效液相色谱法测定大鼠血浆中倍半萜内酯类化合物苍耳亭的浓度[J].中国医院用药评价与分析,2020,20(12):1417-1420. DOI： 10.14009/j.issn.1672-2124.2020.12.003.

CAO Xiu, HAN Jingwen, WU Yan, et al. Determination of Concentration of Xanthine in Rat Plasma by High Performance Liquid Chromatography[J]. 2020,20(12):1417-1420. DOI： 10.14009/j.issn.1672-2124.2020.12.003.

摘要

目的:建立高效液相色谱法测定大鼠血浆中苍耳亭的浓度。方法:采用高效液相色谱法

色谱柱为Hypersil ODS2柱（250 mm×4.6 mm

5μm）

流动相为甲醇-水（V∶V=52∶48）

流速为1.0 ml/min

检测波长为278 nm

柱温为25℃。血浆样品经二氯甲烷萃取富集

10μl进样检测。结果:苍耳亭质量浓度在0.1～10μg/ml（R

=0.999 7）范围内呈现良好的线性关系

定量下限为0.1μg/ml。苍耳亭低、中及高浓度的提取回收率分别为89.19%、83.56%及96.72%

日内和日间精密度分别≤5.47%和8.65%。结论:本方法灵敏快速简单

适用于体内苍耳亭的浓度分析

为药动学研究提供方法依据。

Abstract

OBJECTIVE:To establish the high performance liquid chromatography（HPLC） method for determination of concentration of xanthine in rat plasma.METHODS:HPLC methods was adopted with Hypersil ODS2 column（250 mm × 4.6 mm

5 μm）.The mobile phase was methanol-water（V:V=52:48）

the flow rate was1.0 ml/min

the detection wavelength was 278 nm

and the column temperature was 25 ℃.The plasma samples were extracted and enriched with dichloromethane

and 10 μl injection was used for detection.RESULTS:The calibration curve of xanthatin in plasma showed good linear correlation in the range from 0.1 to 10 μg/ml（R

=0.999 7） and the lower limit of concentration was 0.1 μg/ml.The extraction recovery rates of low

medium and high concentration of Xanthatin was respectively 89.19%

83.56% and 96.72%

and the intra-day and inter-day precision was respectively≤5.47% and 8.65%.CONCLUSIONS:This method is sensitive

rapid and simple

and is suitable for the analysis of the concentration of xanthine in vivo

and provides basis for pharmacokinetic research.

关键词

苍耳亭高效液相色谱法血药浓度大鼠

Keywords

XanthatinHigh performance liquid chromatographyPlasma concentrationRat

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