右美托咪定对肺癌A549细胞炎症、凋亡及化疗敏感性的研究

张强; 邱德亮; 彭英; 董碧倩

doi:10.14009/j.issn.1672-2124.2020.11.008

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右美托咪定对肺癌A549细胞炎症、凋亡及化疗敏感性的研究

论著 | 更新时间：2022-10-08

- 右美托咪定对肺癌A549细胞炎症、凋亡及化疗敏感性的研究
- Effects of Dexmedetomidine on Inflammation,Apoptosis and Chemotherapy Sensitivity of Lung Cancer A549 Cells
- 中国医院用药评价与分析 2020年20卷第11期页码：1312-1315
- 作者机构：
  
  1. 成都市龙泉驿区第一人民医院麻醉科
  2. 川北医学院附属医院麻醉科
- 作者简介：
- 基金信息：
  
  四川省教育厅2018年度科研计划项目（No.18ZA0209）
- DOI：10.14009/j.issn.1672-2124.2020.11.008
  中图分类号： R965
- 纸质出版日期：2020，
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[1]张强,邱德亮,彭英,董碧倩.右美托咪定对肺癌A549细胞炎症、凋亡及化疗敏感性的研究[J].中国医院用药评价与分析,2020,20(11):1312-1315+1320.

ZHANG Qiang, QIU Deliang, PENG Ying, et al. Effects of Dexmedetomidine on Inflammation,Apoptosis and Chemotherapy Sensitivity of Lung Cancer A549 Cells[J]. 2020,20(11):1312-1315.
[1]张强,邱德亮,彭英,董碧倩.右美托咪定对肺癌A549细胞炎症、凋亡及化疗敏感性的研究[J].中国医院用药评价与分析,2020,20(11):1312-1315+1320. DOI： 10.14009/j.issn.1672-2124.2020.11.008.

ZHANG Qiang, QIU Deliang, PENG Ying, et al. Effects of Dexmedetomidine on Inflammation,Apoptosis and Chemotherapy Sensitivity of Lung Cancer A549 Cells[J]. 2020,20(11):1312-1315. DOI： 10.14009/j.issn.1672-2124.2020.11.008.

摘要

目的:探讨右美托咪定对肺癌A549细胞炎症、凋亡及化疗敏感性的影响。方法:设A549细胞组、5-氟尿嘧啶组（40.0μg/ml）、右美托咪定组（80.0μg/ml）及联合组（5-氟尿嘧啶40.0μg/ml+右美托咪定80.0μg/ml）;上述各组细胞置于CO

培养箱（37℃、5%CO

、2%O

、93%N2）。以上各组每孔设6个平行样

培养72 h。培养结束后

以酶联免疫吸附法测定A549细胞炎症因子白细胞介素2（IL-2）、白细胞介素6（IL-6）及肿瘤坏死因子α（TNF-α）蛋白水平

以噻唑蓝（MTT）法及结晶紫染色测定细胞活力及单克隆形成数目

采用流式细胞仪测定凋亡水平

采用Matrigel膜测定侵袭迁移水平

以实时荧光定量PCR（qRT-PCR）法及蛋白免疫印迹法测定核因子κB（NF-κB）、原癌基因（c-myc） mRNA和蛋白水平。结果:与A549细胞组比较

5-氟尿嘧啶组、右美托咪定组及联合组细胞IL-2、IL-6、TNF-α蛋白水平

吸光度

存活率

NF-κB

c-myc mRNA和蛋白表达水平明显降低

细胞克隆形成数目、穿膜数明显减少

凋亡率明显升高

差异均有统计学意义（P

0.05）。与5-氟尿嘧啶组、右美托咪定组比较

联合组细胞IL-2、IL-6、TNF-α蛋白水平

吸光度

存活率

NF-κB

c-myc mRNA和蛋白表达水平明显降低

细胞克隆形成数目、穿膜数明显减少

凋亡率明显升高

差异均有统计学意义（P

0.05）。结论:右美托咪定能抑制肺癌A549细胞炎症、增殖和侵袭

促进其凋亡

增加5-氟尿嘧啶对肺癌A549细胞的化疗作用;其机制与右美托咪定能增强5-氟尿嘧啶对A549细胞NF-κB、c-myc mRNA和蛋白表达的抑制作用有关。

Abstract

OBJECTIVE: To investigate the effects of dexmedetomidine on inflammation

apoptosis and chemotherapy sensitivity of lung cancer A549 cells. METHODS: A549 cells group

5-fluorouracil group（ 40. 0 μg/ml）

dexmedetomidine group（ 80. 0 μg/ml）

combination group（ 40. 0 μg/ml of 5-fluorouracil + 80. 0 μg/ml of dexmedetomidine） were set up. The above cells were placed in CO

incubator（ 37 ℃

5% CO

2% O

93% N2）.Six parallel samples were set in each hole of each group for 72 h. At the end of culture

the levels of interleukin-2（ IL-2）

interleukin-6（ IL-6）

tumor necrosis factor-α（ TNF-α） protein in A549 cells were measured by enzyme linked immunosorbent assay

cell viability and number of monoclonal formation were measured by MTT and crystal violet staining

apoptosis was measured by flow cytometry

invasion and migration was measured by Matrigel membrane

nuclear factor-κB（ NF-κB）

c-myc mRNA and protein were measured by quantitative real time fluorescent PCR（ qRT-PCR） and Western Blot. RESULTS: Compared with A549 cell group

the levels of IL-2

IL-6

TNF-αprotein

optical delnsity（ OD）

survival rate

number of cell clone formation

number of membrane penetration

NF-κB

c-myc mRNA and protein expression in 5-fluorouracil group

dexmedetomidine group and combination group decreased significantly

the apoptosis rate increased significantly

the differences were statistically significant（ P

0. 05）.Compared with 5-fluorouracil group and dexmedetomidine group

the levels of IL-2

IL-6

TNF-α protein

survival rate

number of cell clone formation

number of membrane penetration

NF-κB

c-myc mRNA and protein expression in the combined group decreased significantly

the apoptosis rate increased significantly

the differences were statistically significant（ P

0. 05）. CONCLUSIONS: Dexmedetomidine can inhibit the inflammation

proliferation and invasion of lung cancer A549 cells

promote the apoptosis and increase the chemotherapy effects of 5-fluorouracil on lung cancer A549 cells. The mechanism is related to that dexmedetomidine can enhance the inhibition of 5-fluorouracil on the expression of NF-κB

c-myc mRNA and protein in A549 cells.

关键词

右美托咪定肺癌炎症凋亡化疗敏感性

Keywords

DexmedetomidineLung cancerInflammationApoptosisChemotherapy sensitivity

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