OBJECTIVE: To establish a method of reversed phase high performance liquid chromatography （RPHPLC） for the determination of teicoplanin in human plasma. METHODS: Waters C

18

column （150 mm × 4. 6 mm

5 μm） was used

the mobile phase was 0. 02 mol/L of NaH

2

PO

4

（pH = 2. 50）-acetonitrile （V ∶V = 74. 5 ∶25. 5）

the internal standard substance was methyl parahydroxybenzoats

with the flow rate was 1. 0 ml/min

the detection wavelength was 240 nm

the injection volume was 0. 020 ml and the column temperature was 35 ℃ . RESULTS: Under the above chromatographic conditions

both the resolutions of teicoplanin and internal standard substance were good （R

>

1. 5）

the retention time of teicoplanin was about 9 min and the retention time of internal standard substance was about 11 min

the linear relationship was good in the range of 3. 125-100 μg/ml （r = 99. 96%

n = 6）; when the lower limit of quantification was 3. 125 μg/ml

the signal-to-noise ratio （S/N） was 18. 5

both the accuracy and precision of intra-batch and inter-batch meet the requirements. CONCLUSIONS: This research method is suitable for determining the concentration of teicoplanin in human plasma and provides guidance for the individualized medication of teicoplanin.