OBJECTIVE: To probe into the effect of prescription of turbidity-resolving and lipid-eliminating on visfatin level

oxidative stress and hepatocyte apoptosis in rats with type 2 diabetes complicated with hyperlipidemia.METHODS: 60 healthy SD rats were selected

after 1-week feeding

6 rats were brought into normal group and 54 rats were brought into model group according to random weight grouping method. The normal group was fed with conventional fodder

while the model group was fed with high-sugar and high-fat fodder. After 8 weeks

rats in the model group were intraperitoneally injected with 2% streptozotocin to prepare rat model of type 2 diabetes complicated with hyperlipidemia

36 rats with significant differences from the normal group were selected for study and divided into model group

metformin group

Zhibituo capsule group and turbidity-resolving and lipid-eliminating group（ low

medium and high dose） via randomized block

with 6 rats in each group. Intragastric administration was given to rats in each group

after 1-month treatment

the visfatin level

oxidative stress [superoxide dismutase（ SOD）

malonaldehyde（ MDA）

advanced glycosylation end products（ AGEs） and advanced oxidation protein products（ AOPP） ]

hepatocyte apoptosis

B-cell lymphoma-2（ Bcl-2） and Bcl-2 associated X protein（ Bax） expression were compared among each group. RESULTS: The visfatin level in model group was significantly higher than other groups

which was statistically different with that of normal group

low

medium and high dose of turbidity-resolving and lipideliminating group（ P < 0. 05）; the SOD level of model group was significantly lower than other groups

while the AGEs

MDA and AOPP levels were significantly higher than other groups

which were statistically different with those of normal group

medium and high dose of turbidity-resolving and lipid-eliminating group（ P<0. 05）

the AOPP level of model group was statistically different with that of low dose of turbidity-resolving and lipid-eliminating group（ P <0. 05）. The cell apoptosis of model group was significantly higher than other group

which was statistically different with that of normal group

metformin group

low and medium dose of turbidity-resolving and lipid-eliminating group（ P<0. 05）. Cell proportion of model group at stationary phase（ G0/G1） was significantly higher than that of normal group

with statistically significant difference（ P< 0. 05）; which was slightly higher than each administration group

with no statistically significant difference（ P > 0. 05）. The proliferation index of model group was significantly lower than that of normal group

with statistically significant difference（ P < 0. 05）; which was slightly lower than each administration group

with no statistically significant difference（ P>0. 05）. The fluorescence index（ FI） of Bax protein expression of model group was significantly higher than that of normal group

low

medium

high dose of turbidityresolving and lipid-eliminating group; the FI of Bcl-2 protein expression was significantly lower than that of normal group

metformin group

low and medium dose of turbidity-resolving and lipid-eliminating group; the expression level of Bax/Bcl-2 protein were significantly higher than that of normal group

metformin group

low

medium and high dose of turbidity-resolving and lipid-eliminating group

with statistically significant differences（ P<0. 05）. CONCLUSIONS:Prescription of turbidity-resolving and lipid-eliminating can effectively reduce the visfatin level in rats with type 2 diabetes complicated with hyperlipidemia

and improve oxidative stress; which can reduce hepatocyte apoptosis by enhancing the expression of Bcl-2 protein and weakening the expression of Bax protein.