OBJECTIVE: To evaluate and validate the method of ultra-high performance liquid chromatography for the determination of teicoplanin in human plasma. METHODS: Plasma samples were pretreated with 90% acetonitrileprecipitated protein

using methyl parahydroxybenzoate as an internal standard

the Waters Symmetry C18 column was adopted and 0. 01 mol/L NaH2 PO4-acetonitrile（ V ∶V = 77 ∶23） was set as the mobile phase; the detection wavelength was 215 nm; the flow rate was 1. 0 ml/min; the column temperature was 35 ℃; the injector temperature was 4 ℃ .RESULTS: Tecolanin had a good linear relationship in the range of 3. 23-103. 25 μg/ml（ r2= 0. 999 7）

the intra-day and inter-day precision measurements showed RSD were less than 15%. CONCLUSIONS: This method is fast

simple

accurate and reliable

with strong specificity and good reproducibility

which can be used for the determination of teicoplanin in plasma.