Objective: To establish the high-performance liquid chromatography combined with quantitative analysis of multi-components by single-marker (HPLC-QAMS) technology for the quantitative control of 8 kinds of components in Xiao’er Kesouning syrup

including mulberroside A

morusin M

dihydromorin

baicalin

wogonoside

praeruptorin A

praeruptorin B and praeruptorin E. Methods: Ultrasil C18 column was used with the mobile phase of acetonitrile (A) and 1 mL/ L glacial acetic acid. The flow rate was 1. 0 mL/ min with a linear gradient elution

detection wavelengths were set at 280 nm ( mulberroside A

morusin M

dihydromorin

baicalin and wogonoside)

and 321 nm (praeruptorin A

praeruptorin B and praeruptorin E)

the column temperature was maintained at 25 ℃. Baicalin was used as the reference substance

relative correction factors for the other 7 kinds of components were established to calculate the content of mulberroside A

morusin M

dihydromorin

wogonoside

praeruptorin A

praeruptorin B and praeruptorin E. Results: A good linear relationship with peak area was observed within the range from 0. 79 to 19. 75 μg / mL for mulberroside A

0. 56 to 14. 00 μg / mL for moracin M

0. 38 to 9. 50 μg / mL for dihydromorin

26. 55 to 663. 75 μg / mL for baicalin

4. 66 to 116. 50 μg / mL for wogonoside

5. 87 to 146. 75 μg / mL for praeruptorin A

1. 71 to 42. 75 μg / mL for praeruptorin B and 2. 39 to 59. 75 μg / mL for praeruptorin E

respectively. The average recovery rates for each component at low

medium and high concentrations ranged from 96. 83% to 100. 14%

with relative standard deviation ( RSD) ≤1. 42% ( n = 9). The results obtained by using the HPLC-QAMS technology showed no significant difference from those obtained by using the external standard method (ESM). Conclusion: HPLC-QAMS technology provides a fast and feasible multi-marker simultaneous quality evaluation model for Xiao’er Kesouning syrup.