Li Sai, Li Shoulin, Su Lingying. Determination of Caffeine Blood Concentration in Neonates by High Performance Liquid Chromatography and Its Clinical Application[J]. 2023,29(8):31-34.
Li Sai, Li Shoulin, Su Lingying. Determination of Caffeine Blood Concentration in Neonates by High Performance Liquid Chromatography and Its Clinical Application[J]. 2023,29(8):31-34. DOI: 10.13407/j.cnki.jpp.1672-108X.2023.08.009.
Objective: To establish the high performance liquid chromatography (HPLC) method for the determination of caffeine blood concentration in neonates
and to determine the concentration of caffeine in neonates with primary apnea. Methods: The determination was performed on ZORBAX Extend C18 column with mobile phase acetonitrile-water (19 ∶ 81
V/ V) at the column temperature of 25 ℃ and flow rate of 1 mL/ min. The detection wavelength was 273 nm. Results: Caffeine blood concentration showed good linearity ( r =0. 998
9) in the range of 1. 0 to 160. 0 μg / mL
with mean recoveries of 98. 40% to 102. 40%. Inter-day and intra-day relative standard deviation (RSD) was ≤9. 63%. The average caffeine blood concentration in 10 children was ( 20. 53± 11. 12) μg / mL after 7 d ofadministration. Conclusion: The established high performance liquid chromatography method is accurate
rapid and validated for the determination of caffeine blood concentration in neonates.