Objective: To explore the mechanism of the core drugs of Changju Zhidong prescription in the treatment of tic disorders (TD) by network pharmacology. Methods: Based on literature research and retrieval of Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP)

we obtained the active ingredients of the core drugs in Changju Zhidong prescription

and predicted their targets. Drugbank

Genecards

OMIM

DisGeNET and TTD databases were used to search TD-related target genes

and they were intersected with the drug targets. Cytoscape 3. 7. 2 was used to construct the “drug-active ingredientstargets” network and conduct topological analysis. STRING database was used to construct the protein-protein interaction network and screen out the key targets. GO biological process analysis and KEGG pathway enrichment analysis were performed based on DAVID. AutoDock Vina and Pymol were used to dock the molecules of the key ingredients with the key targets. Results: A total of 81 active ingredients and 283 related potential targets were screened out. A total of 2􀆯 250 TD-related target genes were screened out

125 key targets were extracted

and 18 core targets were screened out

including protein kinase B1 (AKT1)

interleukin-6 (IL-6)

tumor necrosis factor (TNF)

interleukin-1β (IL-1β) and estrogen receptor 1 (ESR1). Common targets were mainly concentrated in neuroactive ligand-receptor interaction

calcium signaling pathway

T cell receptor signaling pathway and other pathways

which were related with response to estradiol

chemical synaptic transmission

and immune response. The results of molecular docking showed that the key ingredients have a good binding activity with the key targets. Conclusion: The core drugs of Changju Zhidong prescription play a therapeutic role on TD through multiple ingredients

multiple targets

and multiple pathways regulation.