Objective: To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous content determination of four constituents in Baoerning granules. Methods: Zorbax Eclipse-C18 (250 mm×4. 6 mm

5 μm)was used as stationary phase

acetonitrile-0. 2% phosphoric acid solution with gradient elution (0 min→14 min→15 min

acetonitrile 15%→25%→ 15%) at a flow rate of 1. 0 mL/ min

the column temperature at 32 ℃

and the wavelength of detection at 215 nm. Prim-Oglucosylcimifugin was chosen as the single maker to determine the contents of pistil isoflavone glucoside

cimifugin and 5-Omethylvisammioside in Baoerning granules. At the same time

the external standard method was used to determine the contents of four compinents in Baoerning granules

and the differences between the two methods were compared to verify the accuracy and feasibility of the QAMS method. Results: The relative correction factors have good reproducibility. There was a good linear relationship (r≥0. 999

4) in the concentration range of the four compounds in the external standard method. The recoveries were 99. 21% to 100. 95% (n = 6). There was no significant difference between the content value calculated by the correction factor and the measured value. Conclusion: This method is accurate and reliable

and can be used for the quality control of Baoerning granules