Objective: To establish the determination method of loganin

sweroside

secoxyloganin

liquiritin

glycyrrhizic acid

forsythoside B

forsythoside A and forsythin in Xiao’er Qingre Huatan suppository based on high performance liquid chromatography-quantitative analysis of multi-components by single-marker (HPLC-QAMS) method. Methods: Agilent Zorbax SB-C18 was adopted

acetonitrile-0.2% phosphoric acid solution was the mobile phase by gradient elution. The detection wavelength was set at 237 nm for loganin

sweroside

secoxyloganin

liquiritin and glycyrrhizic acid

and 275 nm for forsythoside B

forsythoside A and forsythin. The flow rate was 1.0 mL/min at column temperature of 30 ℃. Liquiritin was used as an internal standard

the relative correction factors (RCF) of loganin

sweroside

secoxyloganin

glycyrrhizic acid

forsythoside B

forsythoside A and forsythin were calculated. The method was validated by comparison of the quantitative results between external standard method (ESM) and HPLC-QAMS method. Results: Loganin

sweroside

secoxyloganin

liquiritin

glycyrrhizic acid

forsythoside B

forsythoside A and forsythin showed good linear relationships within the ranges from 4.08 to 102.00

from 0.77 to 19.25

from 4.59 to 114.75

from 1.35 to 33.75

from 2.74 to 68.50

from 1.61 to 40.25

from 7.73 to 193.25 and from 2.19 to 54.75 μg/mL (r≥0.999

1)

the average recoveries and RSD were respectively 99.45% (0.96%)

97.49% (1.45%)

98.85% (1.08%)

99.13% (0.78%)

100.04% (0.76%)

98.63% (1.59%)

100.07% (0.60%) and 96.99% (1.26%). There was no significant difference between the contents calculated by HPLC-QAMS and measured by external standard method for each component in Xiao’er Qingre Huatan suppository. Conclusion: The HPLC-QAMS method is simple with lower cost

and can be used for the multi-index component quality evaluation of Xiao’er Qingre Huatan suppository.