Objective To investigate the changes of Miat and Wnt7b/β-catenin signaling pathway in hippocampal neurogenesis of immature rats with temporal lobe epilepsy. Methods: Immature rats aged from 21 to 23 d were divided into the normal saline (NS) group and the kainic acid (KA) group

with 18 rats in each group. The temporal lobe epilepsy model was established by intracerebroventricular injection of KA in the KA group

and the electrode of electroencephalography was embedded. The epilepsy and electroencephalography changes of the immature rats were observed. At the 1st

4th

and 8th weeks

the learning and memory abilities of the immature rats were tested by Y-maze. The brain tissues of immature rats were collected

and the number of BrdU positive cells and expression of DCX protein were detected after embedding sections. Hippocampal tissues of immature rats were collected

RNA was extracted to detect the content of Miat

and protein was extracted to detect the expression of Wnt7b/β-catenin signaling protein. Results: The immature rats in KA group had epileptic seizures at different periods

and the electroencephalography showed continuous high-frequency/high-amplitude spikes. As time goes on

compared with the epilepsy frequency (7.50±1.87) times/day and epilepsy grade (3.31±0.48) in the KA-1 w group

the epilepsy frequency (2.33±1.21) times/day and epilepsy grade (1.19±0.40) in the KA-8 w group decreased (P<0.05)

and high-frequency/high-amplitude spikes appeared intermittently in brain waves. In the Y-maze

compared with the NS group

the number of correct response of KA group decreased

and the correct response duration prolonged. Compared with the number of correct response (8.17±1.72) times and correct response duration (11.83±1.72) s in the KA-1 w group

the number of correct response (10.50±2.74) times in the KA-8 w group increased

the correct response duration (9.50±2.74) s shortened. Compared with the NS group

the number of BrdU positive cells

DCX protein expression

Miat content

Wnt7b/β-catenin signaling pathway and BDNF/TrkB signaling pathway protein expression in the KA group decreased (P<0.05). Compared with the KA-1 w group

the number of BrdU positive cells

DCX protein expression

Miat content

Wnt7b/β-catenin signaling pathway and BDNF/TrkB signaling pathway protein expression in the KA-8 w group increased (P<0.05). Conclusion: There is a decrease in neurogenesis in the hippocampus of immature mice with temporal lobe epilepsy

which may be related to the inhibition of Miat and Wnt7b/β-catenin signaling pathway.