Objective: To establish the method for simultaneous determination of 6 preservatives including sodium benzoate in Feilike mixture by high performance liquid chromatography (HPLC). Methods: HPLC was performed on Agilent XDB-C18 column (250 mm×4.6 mm

5 μm) with mobile phase of methanol-0.025 mol/L sodium dihydrogen phosphate solution (gradient elution). The flow rate was 1.0 mL/min. The column temperature was 30 ℃

and the detection wavelength was 228 nm and 255 nm. Results: There were good linearity in the range from 0.063

54 to 211.800

00 μg (r=0.999

9) for sodium benzoate

from 0.066

06 to 220.200

00 μg (r=0.999

9) for potassium sorbate

from 0.062

70 to 209.000

00 μg (r=0.999

9) for methyl p-hydroxybenzoate

from 0.0661

2 to 220.400

00 μg (r=0.999

9) for ethyl p-hydroxybenzoate

from 0.067

98 to 226.600

00 μg (r=0.999

9) for propyl p-hydroxybenzoate

from 0.063

30 to 211.000

00 μg (r=0.999

9) for butyl p-hydroxybenzoate. The average recoveries were 100.72% (RSD=0.82%)

104.82% (RSD=1.59%)

101.11% (RSD=0.25%)

102.39% (RSD=1.33%)

102.56% (RSD=1.35%) and 101.09% (RSD=0.34%)

respectively. Sodium benzoate

methyl p-hydroxybenzoate and butyl p-hydroxybenzoate were not detected in 10 batches of samples

and the contents of sodium benzoate

ethyl p-hydroxybenzoate and propyl p-hydroxybenzoate were respectively from 2.132

6 to 2.602 6 mg/mL

from 0.110

8 to 0.126

0 mg/mL

from 0.399

9 to 0.408

9 mg/mL. Conclusion: The method has the advantages of simple operation

strong specificity and high accuracy

and can comprehensively control the content of preservatives in Feilike mixture.