Objective: To probe into the effects of microRNA-515-5p (miR-515-5p) on proliferation

invasion

migration and molecular mechanisms of SK-N-BE(2) and SK-N-AS of neuroblastoma (NB) cells. Methods: Bioinformatics techniques combined with multiple software prediction methods were used to detect the expression of miR-515-5p in NB. The miR-515-5p mimics were transiently transfected in NB cells

and effects of miR-515-5p on the proliferation

invasion and migration of NB cells after transfection were examined by CCK8 method and colony-forming unit assay

Transwell invasion cell assay and in vitro scratch assay. Western Blot was used to detect the regulatory mechanism of miR-515-5p on the c-MYC-p53 signal axis. Results: Compared with the control group

cells transfected with miR-515-5p mimics had the reduced mean colony formation rate

decreased cell activity

and reduced cell invasion and migration ability (P<0.05). Western Blot showed that miR-515-5p inhibited the expression of c-MYC protein

yet promoted the activation of P-p53 (P<0.05)

and had no effect on the expression of p53. Conclusion: The miR-515-5p inhibits the proliferation

invasion and migration of NB cells by regulating the c-MYC-p53 signaling axis

which may be the potential target for molecular therapy of NB.