Objective: To quantitative analyze the main components in Xiao’er Kechuanling oral solution. Methods: The determination methods for ephedrine hydrochloride

pseudoephedrine hydrochloride and asarinin in Xiao’er Kechuanling oral solution were established by high performance liquid chromatography-diode array detector (HPLC-DAD). Results: Ephedrine hydrochloride

pseudoephedrine hydrochloride and asarinin were well separated under the maximum absorption wavelength. Both the degree of separation and linear relationship met the requirements of Chinese Pharmacopoeia. Ephedrine hydrochloride was linear within the range from 0.009

3 to 0.093

0 μg/mL (r=0.999

9). Pseudoephedrine hydrochloride was linear within the range from 0.002

9 to 0.029

0 mg/mL (r=0.999

8). Asarinin was linear within the range from 0.6 to 6.0 μg/mL (r＝0.999

8). Conclusion: The method is simple

reproducible with lower cost

and can be used for the quality control of Xiao’er Kechuanling oral solution in hospital preparation room.