Objective: To probe into the application of pyridostigmine bromide and levodopa in growth hormone (GH) provocative test and the peak time of GH consistence

so as to further reduce the pain caused by repeated blood collection in children. Methods: Totally 88 children of short stature with provocative test of pyridostigmine bromide and levodopa admitted into endocrinology department of our hospital from Jan. to Aug. 2018 were extracted. The peak time of GH consistence was analyzed. Results: The peak time of GH consistence occurred in 69 cases (78.41%) after 30 to 60 min of drug combination

among which 40 cases (45.45%) showed the peak after 60 min

while only 14 cases (15.91%) showed the peak after 90 to 120 min

the difference was statistically significant (χ2=68.97

P<0.01). Conclusion: In the combined provocation test of pyridostigmine bromide and levodopa

more cases would reach the peak time of GH consistence after application from 30 to 60 min and less after application from 90 to 120 min. Therefore

intravenous blood collection should be reduced after treatment of 120 min

which can be applied in clinical practice.