Objective: To establish the microbial limit test method for Zihuang ointment of hospital preparation. Methods: By reference to Chinese Pharmacopoeia (2015 Edition)

the total number of aerobic bacteria was determined by the dilution method of the test solution (1∶100). The total number of mold and yeasts were determined by the pouring plate method. The control bacteria of Staphylococcus aureus and Pseudomonas aeruginosa were examined by using the test solution dilution method (100 mL). Results: The recovery ratios of the total aerobic bacteria

mold and yeasts experientmental bacteria were all within the range from 0.5 to 2.0. S. aureus and P. aeruginosa showed good growth in the experimental group

but no growth in the negative control group and the sample group. Conclusion: The method established in this experiment has been verified by methodology

the results are in line with the specifications of the Chinese Pharmacopoeia (2015 Edition)

which can provide the scientific basis for the revision of the quality standard of Zihuang ointment of hospital preparation.