Objective: To observe the expression of octamer-binding transcription factor 4 (Oct4) in neuroblastoma

and probe into its correlation with metastasis and invasion of neuroblastoma. Methods: The expression of Oct4 in neuroblastoma tissues and paracancerous tissues was detected by immunohistochemistry and real-time PCR (RT-PCR). The clinicopathological parameters of the patients were collected

and the correlation between expression of Oct4 and clinicopathological parameters of neuroblastoma was analyzed. The electroporation transfection method was used to transfer Oct4 siRNA into neuroblastoma SK-N-MC cells. Effects of Oct4 on metastasis and invasion of neuroblastoma SK-N-MC cells was detected by wound healing assay and Boyden chamber. Results: The expression level of Oct4 in neuroblastoma tissues was higher than that in paracancerous tissues (P<0.05). The positive expression rate of Oct4 was closely related to the presence or absence of lymph node metastasis and differentiation (P<0.01)

but it is not significantly related to patient’s gender

age

tumor size and clinical pathological stage (P>0.05). Transfection of Oct4 siRNA can significantly inhibit the metastasis and invasion of neuroblastoma SK-N-MC cells (P<0.01). Conclusion: Oct4 can promote the metastasis and invasion of neuroblastoma

and its high expression may be closely related to the occurrence and development of neuroblastoma.