Objective:To evaluate the clinical value of measuring the serum levels of pepsinogen Ⅰ(PGⅠ)

pepsinogen Ⅱ(PGⅡ)and double staining in 446 patients with gastric precancerous lesions or gastric carcinoma.Methods:PGⅠ

PGⅡ and PG Ⅰ/PGⅡ ratio(PGR) were measured with the two-site"sandwich"technique of enzyme-linked immunosorbent assay(ELISA) in these 446 patients.After that

these patients were randomized into two groups

223 patients were double stained with indigo carmine-methylene blue

while another 223 patients were not stained.At last

all of them were confirmed with pathology.Results:The serum PG Ⅰ

PGR level of chronic atrophic gastritis(CAG) patients and gastric carcinoma(GC) patients were significantly lower than that of chronic superficial gastritis(CSG) patients

gastric ulcer(GU) patients and healthy group(P<0.01).The PG Ⅰ level of gastric ulcer patients was significantly higher than that of the healthy group and the other groups(P<0.01).The PGR in CAG and GC patients was significantly lower than that in the other groups(P<0.01).In double stained group

the prevalence rate was 72.20%.In nonstained group

the prevalence rate was 53.36%.Conclusion:Feasibility and the necessity of combined determination were evaluated.Compared with solo determination

for patients with gastric lesions

it was more sensitive to determine and analyze the serum levels of PG Ⅰ

PG Ⅱ and double staining.So it is recommended to popularize the combined determination of serum PG Ⅰ

PGⅡ and double staining in clinica1 distinctive diagnosis of gastric precancerous lesions.