TGF-β<sub>1</sub>和α-SMA在大鼠皮层注射FeCl<sub>3</sub>所致痫性发作中的表达

陶武; 谢延风; 石全红; 詹彦; 张光伟; 王佳

doi:10.13406/j.cnki.cyxb.2011.09.026

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TGF-β₁和α-SMA在大鼠皮层注射FeCl₃所致痫性发作中的表达

基础研究 | 更新时间：2024-01-17

- TGF-β₁和α-SMA在大鼠皮层注射FeCl₃所致痫性发作中的表达
- The expression of TGF-β₁ and α-SMA between young and adult rats after intracortical injection of ferric chloride
- 重庆医科大学学报 2011年36卷第9期页码：1066-1069
- 作者机构：
  
  1. 重庆医科大学附属永川医院神经外科
  2. 重庆医科大学附属第一医院神经外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.09.026
  中图分类号： R742.1
- 纸质出版日期：2011，
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[1]陶武,谢延风,石全红,詹彦,张光伟,王佳.TGF-β_1和α-SMA在大鼠皮层注射FeCl_3所致痫性发作中的表达[J].重庆医科大学学报,2011,36(09):1066-1069.

TAO Wu, XIE Yan-feng, SHI Quan-hong, et al. The expression of TGF-β₁ and α-SMA between young and adult rats after intracortical injection of ferric chloride[J]. Journal of Chongqing Medical University, 2011,36(9):1066-1069.
[1]陶武,谢延风,石全红,詹彦,张光伟,王佳.TGF-β_1和α-SMA在大鼠皮层注射FeCl_3所致痫性发作中的表达[J].重庆医科大学学报,2011,36(09):1066-1069. DOI： 10.13406/j.cnki.cyxb.2011.09.026.

TAO Wu, XIE Yan-feng, SHI Quan-hong, et al. The expression of TGF-β₁ and α-SMA between young and adult rats after intracortical injection of ferric chloride[J]. Journal of Chongqing Medical University, 2011,36(9):1066-1069. DOI： 10.13406/j.cnki.cyxb.2011.09.026.

摘要

目的:研究转化生长因子β1（Transforming growth factor beta 1

TGF-β1）和α-平滑肌肌动蛋白（Alpha-smooth muscleaction

α-SMA）的表达差异与幼年和成年大鼠皮层注射FeCl3所致痫性发作的相关性

探讨其在大鼠痫性发作中的可能机制。方法:分别用FeCl3和生理盐水（Normal saline

N.S）注射幼年与成年大鼠皮层

观察大鼠痫性发作情况。ELISA法检测脑脊液（Cerebral spinal fluid

CSF）中TGF-β1变化

RT-PCR和Western免疫印迹法检测注射区域α-SMA表达。结果:只有幼鼠FeCl3组出现癫痫样发作;TGF-β1和α-SMA在幼鼠FeCl3组表达最高。结论:幼年比成年鼠更易并发癫痫发作

且TGF-β1和α-SMA表达更高

推断TGF-β1和α-SMA表达增加是幼鼠更易并发痫性发作的重要机制之一。

Abstract

Objective:To study the correlation between expressions of TGF-β1 & α-SMA and seizures of rats after intracortical injection of ferric chloride and explore its role in occurrence of rats seizure.Methods:Rats(childhood and adulthood) were treated with an intracortical injection of FeCl3 and normal saline

the occurrence of seizures were under observation;TGF-β1 in cerebral spinal fluid(CSF)was tested by ELISA;the expressions of mRNA and protein of α-SMA were detected by RT-PCR and Western blotting.Results:Epilepsy seizure was only observed in FeCl3 childhood group;TGF-β1 and α-SMA expression ranks top 1 in FeCl3 childhood group.Conclusion:Childhood rats were more susceptible to seizure than adulthood rats with higher level of TGF-β1 and α-SMA expressions;implying that the growth of TGF-β1 and α-SMA expressions might be the one of key mechanisms in seizure.

关键词

癫痫发作转化生长因子β1α-平滑肌肌动蛋白

Keywords

epilepsy seizureTGF-β1α-SMA

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TGF-β1和α-SMA在大鼠皮层注射FeCl3所致痫性发作中的表达

The expression of TGF-β1 and α-SMA between young and adult rats after intracortical injection of ferric chloride

DOI：10.13406/j.cnki.cyxb.2011.09.026

摘要

Abstract

关键词

Keywords

references

TGF-β₁和α-SMA在大鼠皮层注射FeCl₃所致痫性发作中的表达

The expression of TGF-β₁ and α-SMA between young and adult rats after intracortical injection of ferric chloride