Objective:To investigate the relationship between nonalcoholic fatty liver disease(NAFLD) and cholecystolithiasis.Methods:Ultrasonography was used to diagnose NAFLD in 4 076 subjects attending routine health examination in the People’s Hospital of Hunan Province from April 2006 to January 2010.Total 2 830 subjects were enrolled in this study excluding those with excessive drinking

hepatitis B markers

and severe infection

and whose data to diagnose metabolic syndrome were missing.There were 1367 patients who received the routine health examination again during 3 years.The result of ultrasonography was recorded.Results: ①In the subjects undergoing routine health examination in People’s Hospital of Hunan Province

the prevalence of NAFLD in patients with cholecystolithiasis was significantly higher than that in those without cholecystolithiasis(43.20% vs 33.76%

P<0.05).Similarly

the prevalence of cholecystolithiasis in patients with NAFLD was also higher than that in those without NAFLD(11.03% vs.7.67%

P<0.05).②In a following up of 1 367 patients for 1 to 3 years

we found that the morbidity of cholecystolithiasis in patients with NAFLD(n=430) was significantly higher than that in those without NAFLD(n=430)(3.72% vs.1.68%

P<0.05).The presence of NAFLD was associated with an increased risk of cholecystolithiasis(OR=2.264;95% CI

1.094-4.683;P<0.05).Conclusion:①Cholecystolithiasis and NAFLD frequently coexist.②NAFLD may increase the risk of cholecystolithiasis.