Objective:To construct lentivirus vector encoding human apurinic/apyrimidinic endonuclease-1(APE1) gene and examine its ability to express the APE1 gene in vitro.Methods:The specific APE1 sequence was cloned into the plasmid of LV to construct the APE1 expression plasmid LV-APE1.The recombinant plasmids were identified by DNA sequencing and restriction digestion.Then the packaging cell lines(293T cell)were cotransfected with the LV-APE1 together with the plasmids pRsv-REV

pMDlg-PRRE

PMD2G and interfered by phosphate-calcium deposit method.The recombinant lentivirus vector was used to infect the Wistar rat neuron cell.The expression of APE1 was detected by real-time quantitative PCR and Western blot.Results:The results showed the recombinant lentivirus vector carrying APE1 gene was constructed successfully;the virus titers were above 1.0×109 TU/ml

and its infection efficiency in the Wistar rat neuron cell was 100%.The recombinant lentivirus vector could up-regulate the expression of the APE1 gene.Conclusion:The lentivirus vector carrying APE1 gene can enhance the expression of APE1 gene significantly

which lays the basis for its application in the treatment of neurodegenerative disease.