硫化氢对大鼠MIRI心肌细胞凋亡的影响及机制研究

李钢; 杨双强

doi:10.13406/j.cnki.cyxb.2011.08.041

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硫化氢对大鼠MIRI心肌细胞凋亡的影响及机制研究

基础研究 | 更新时间：2024-01-17

- 硫化氢对大鼠MIRI心肌细胞凋亡的影响及机制研究
- Effect and mechanism of hydrogen sulfide on MIRI apoptosis in rat hearts
- 重庆医科大学学报 2011年36卷第8期页码：932-936
- 作者机构：
  
  重庆医科大学附属第一医院胸心外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.08.041
  中图分类号： R965
- 纸质出版日期：2011，
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[1]李钢,杨双强.硫化氢对大鼠MIRI心肌细胞凋亡的影响及机制研究[J].重庆医科大学学报,2011,36(08):932-936.

LI Gang, YANG Shuang-qiang. Effect and mechanism of hydrogen sulfide on MIRI apoptosis in rat hearts[J]. Journal of Chongqing Medical University, 2011,36(8):932-936.
[1]李钢,杨双强.硫化氢对大鼠MIRI心肌细胞凋亡的影响及机制研究[J].重庆医科大学学报,2011,36(08):932-936. DOI： 10.13406/j.cnki.cyxb.2011.08.041.

LI Gang, YANG Shuang-qiang. Effect and mechanism of hydrogen sulfide on MIRI apoptosis in rat hearts[J]. Journal of Chongqing Medical University, 2011,36(8):932-936. DOI： 10.13406/j.cnki.cyxb.2011.08.041.

摘要

目的:探讨H2S后处理对大鼠心肌缺血-再灌注细胞凋亡及缺氧诱导因子-1α（Hypoxia-inducible factor-1alpha

HIF-1α）/血管内皮生长因子（Vascular endothelial growth factor

VEGF）的影响。方法:以硫氢化钠（Sodium hydrosulfide

NaHS）作为外源性H2S供体。健康雄性SD大鼠24只

随机平均分为3组:假手术（Sham）组、缺血再灌注（Ischemia-reperfusion

IR）组、H2S后处理组。用TUNEL法检测心肌细胞凋亡指数（Apoptotic index

AI）

RT-PCR法检测心肌组织HIF-1α和VEGF mRNA表达

Western blot检测HIF-1α和VEGF蛋白的表达情况。结果:相对于IR组

H2S组心肌细胞AI明显下降（P<0.01）

HIF-1αmRNA及蛋白表达水平明显增加（分别为P<0.01和P<0.05）

VEGF mRNA和蛋白表达水平亦显著增加（均为P<0.01）。结论:H2S后处理能促进HIF-1α/VEGF的高表达

对心肌缺血-再灌注损伤（Myocardial ischemia-reperfusion injury

MIRI）诱导的心肌细胞凋亡有抑制作用。

Abstract

Objective:To investigate the effects of hydrogen sulfide postconditioning on myocardial apoptosis and hypoxia-inducible factor-1alpha/vascular endothelial growth factor in a rat model of myocardial ischemia-reperfusion injury.Methods:Sodium hydrosulfide was used as an exogenous donor of hydrogen sulfide.Twenty-four healthy male SD rats were randomly divided into three groups:sham-operation group

simple ischemia-reperfusion group and hydrogen sulfide postconditioning group.The myocardial apoptosis index was detected with TUNEL.Hypoxia-inducible factor-1alpha/vascular endothelial growth factor mRNA were observed by RT-PCR.In addition

the expression of hypoxia-inducible factor-1alpha/vascular endothelial growth factor proteins were measured by Western blot.Results:The myocardial apoptosis index significantly decreased(P<0.01)

and the mRNA and protein expression levels of hypoxia-inducible factor-1alpha(P<0.01

P<0.05

respectively) and vascular endothelial growth factor(all P<0.01) significantly increased in the hydrogen sulfide postconditioning group compared with the simple ischemia-reperfusion group.Conclusion:Hydrogen sulfide postconditioning can promote the high expression of hypoxia-inducible factor-1alpha/vascular endothelial growth factor and inhibit myocardial apoptosis induced by the myocardial ischemia-reperfusion injury.

关键词

硫化氢心肌缺血-再灌注损伤缺氧诱导因子-1α血管内皮生长因子凋亡

Keywords

hydrogen sulfidemyocardial ischemia-reperfusion injuryhypoxia-inducible factor-1alphavascular endothelial growth factorapoptosis

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