4种方法校准血细胞分析仪红细胞压积的结果比较及偏倚分析

杨晏; 王忠诚

doi:10.13406/j.cnki.cyxb.2011.08.015

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4种方法校准血细胞分析仪红细胞压积的结果比较及偏倚分析

技术与方法 | 更新时间：2024-01-17

- 4种方法校准血细胞分析仪红细胞压积的结果比较及偏倚分析
- Comparison on determination results of hematocrit with hematology analyzer using four different calibration methods and its bias analysis
- 重庆医科大学学报 2011年36卷第8期页码：959-962
- 作者机构：
  
  重庆市急救医疗中心检验科
- 作者简介：
- 基金信息：
  
  重庆市卫生局资助项目（编号:05-2-213）
- DOI：10.13406/j.cnki.cyxb.2011.08.015
  中图分类号： R446.11
- 纸质出版日期：2011，
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[1]杨晏,王忠诚.4种方法校准血细胞分析仪红细胞压积的结果比较及偏倚分析[J].重庆医科大学学报,2011,36(08):959-962.

YANG Yan, WANG Zhong-cheng. Comparison on determination results of hematocrit with hematology analyzer using four different calibration methods and its bias analysis[J]. Journal of Chongqing Medical University, 2011,36(8):959-962.
[1]杨晏,王忠诚.4种方法校准血细胞分析仪红细胞压积的结果比较及偏倚分析[J].重庆医科大学学报,2011,36(08):959-962. DOI： 10.13406/j.cnki.cyxb.2011.08.015.

YANG Yan, WANG Zhong-cheng. Comparison on determination results of hematocrit with hematology analyzer using four different calibration methods and its bias analysis[J]. Journal of Chongqing Medical University, 2011,36(8):959-962. DOI： 10.13406/j.cnki.cyxb.2011.08.015.

摘要

目的:评价血红蛋白/平均红细胞血红蛋白浓度（Hemoglobin

Hb/Hean corpuscular hemoglobin concentration

MCHC）比值法（Hb/MCHC法）校准血细胞分析仪红细胞压积（Hematocrit

Hct）的可靠性。方法:分别用Hb/MCHC法、配套校准品、非配套校准品和质控品对3台血细胞分析仪Hct进行校准

分析比较各仪器对Hct为低值、中值和高值的新鲜血标本测定结果的可比性和偏倚。结果:用Hb/MCHC法对新鲜血Hct定值后校准各仪器

3台仪器测定新鲜血Hct结果间的差异均无统计学意义（P>0.05）

偏倚均<1.5%。与Hb/MCHC法校准仪器相比较

2台用配套校准品校准的仪器测定Hct结果间的差异均无统计学意义（P>0.05）

偏倚均<1.0%;无配套校准品的1台仪器测定结果明显增高

其差异有统计学意义（P<0.05）

偏倚达7.14%。用非配套校准品校准的2台仪器测定结果与配套校准品校准仪器的结果差异均有统计学意义（P<0.05）

偏倚分别达6.78%和7.76%。3台用质控品校准的仪器与Hb/MCHC法校准仪器的测定结果差异均有统计学意义（P<0.05）

偏倚达7.56%。结论:血细胞分析仪Hct的校准应使用配套校准品或可靠的定值新鲜血

在配套校准品难于及时获得或缺乏时

Hb/MCHC法可作为新鲜血Hct定值的参考方法。

Abstract

Objective:To evaluate the reliability of hematocrit(Hct) results with hematology analyzer calibrated by Hb/MCHC ratio method.Methods:Three hematology analyzers were calibrated by Hb/MCHC ratio method

manufacturer recommended calibrator

other calibrator and quality control material respectively.The fresh blood sample with low

medium and high value for Hct were detected on each analyzer and the comparability and bias were compared.Results:There were no statistical significant difference in Hct value collected from three analyzer calibrated by the fresh blood measured by Hb/MCHC ratio method(P>0.05)

and the biases of Hct value were all less than 1.5%.Compared with analyzer calibrated by Hb/MCHC ratio method

there were no significant difference among the data collected from two analyzer with recommended calibrator(P>0.05) and the biases were all less than 1.0%.The Hct value collected from the analyzer without recommended calibrator was higher

the bias was 7.14%.Compard with analyzer with recommended calibrator

there were statistical significant difference in Hct value collected from two analyzer after calibration using other calibrator(P<0.05)

and the biases were 6.78% and 7.76%.Compared with analyzer calibrated by Hb/MCHC ratio method

there were significant difference among the data collected from three analyzer after calibration using quality control material(P<0.05) and the bias was 7.56%.Conclusion:The recommended calibrator or accurately measured fresh blood is used to do the calibration of Hct for hematology analyzer.The Hb/MCHC ratio method is a reference method of Hct detection for fresh blood when recommended calibrator is not available or insufficient.

关键词

血细胞分析仪红细胞压积Hb/MCHC比值法校准偏倚

Keywords

hematology analyzerhematocritHb/MCHC ratio methodcalibrationbias

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