Objective:To study the change of CDK13 mRNA expression in the brain tissue of patients with drug-resistant epilepsy so as to explore the possible mechanisms of the pathogenesis of refractory epilepsy.Methods: The microarray scanning and reverse transcription polymerase chain reaction(RT-PCR) were used to evaluate CDK-13mRNA expression in the brain tissue of 40 refractory epileptic patients and 8 normal controls.Results:As compared with the 8 normal controls

there appeared a significant increase in CDK13 mRNA expression in the patients with drug-resistant epilepsy

with the ratio of cy5/cy3 being 2.159 in microarray scanning(CDK13 mRNA expression in the patients with drug-resistant epilepsy was upregulated by 2.159 times compared with the normal controls.)

and the results of RT-PCR were coincided well with those of the cDNA chip.Conclusion: Further study should be made to clarify the precise function of CDK13.CDK13 may take part in the molecular event in neuronal apoptosis as an important modulator.The up-regulation of CDK13 mRNA expression may play an important role in the epileptogenic mechanisms of drug-resistant epilepsy