Objective: To make precise quantitation and distribution analysis of hepatitis B virus(HBV) genotypes in Chongqing.Methods: Real-time genotyping and quantitative PCR(GQ-PCR) was used to genotype and quantitate the HBV strains.Results: A total of 148(97.37%)out of the 152 strains were successfully genotyped.Among them

109 patients(88 males and 21 females) had genotype B with quantity ranging from 4~12 log10copies/ml

and mean and standard deviation of(7.94±1.44) log10copies/ml

10 patients(6 males and 4 females) had genotype C with quantity ranging from 5~10 log10copies/ml

and mean and standard deviation of(8.12±1.64) log10copies/ml

29 patients(24 males and 5 females) had mixtures of genotype B and C with quantity ranging from 4~10 log10copies/ml

and mean and standard deviation of(7.45±1.59) log10copies/ml.The detecting rates of genotype B

genotype C

and genotype B and C were 73.65%(74.58% in males

70.00% in females)

6.76%(5.08% in males

13.30% in females)

and 19.59%(20.34% in males

16.70% in females)

respectively.Conclusion: Genotype B is the most dominant type for HBV strains

followed by mixed genotype B and C infection.Genotype C decreased compared with the previous years.There is no relationship between the gender and HBV genotypes.No significant difference was found between HBV genotype and HBV DNA level.GQ-PCR has a higher sensitivity and specificity for precise simultaneous genotyping and quantifing HBV clinical samples and can replace the traditional genotyping assays.