固体脂质纳米姜黄素联合顺铂对卵巢癌SKOV3的增殖及Bax/Bcl-2蛋白表达的实验研究

邓舒婷; 甘霖; 周琦; 徐建业; 李少林

doi:10.13406/j.cnki.cyxb.2011.06.022

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固体脂质纳米姜黄素联合顺铂对卵巢癌SKOV3的增殖及Bax/Bcl-2蛋白表达的实验研究

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- 固体脂质纳米姜黄素联合顺铂对卵巢癌SKOV3的增殖及Bax/Bcl-2蛋白表达的实验研究
- Effect of curcumin-loaded solid lipid nanoparticles and DDP on proliferation and expression of Bax/Bcl-2 protein in human ovarian cell line SKOV3
- 重庆医科大学学报 2011年36卷第6期页码：650-653
- 作者机构：
  
  1. 重庆医科大学基础医学院放射医学教研室
  2. 重庆市肿瘤研究所妇瘤科
- 作者简介：
- 基金信息：
  
  重庆市卫生局科研资助项目（编号:2007-2-68）
- DOI：10.13406/j.cnki.cyxb.2011.06.022
  中图分类号： R737.31
- 纸质出版日期：2011，
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[1]邓舒婷,甘霖,周琦,徐建业,李少林.固体脂质纳米姜黄素联合顺铂对卵巢癌SKOV3的增殖及Bax/Bcl-2蛋白表达的实验研究[J].重庆医科大学学报,2011,36(06):650-653.

DENG Shu-ting1, GAN Lin2, ZHOU Qi1, et al. Effect of curcumin-loaded solid lipid nanoparticles and DDP on proliferation and expression of Bax/Bcl-2 protein in human ovarian cell line SKOV3[J]. Journal of Chongqing Medical University, 2011,36(6):650-653.
[1]邓舒婷,甘霖,周琦,徐建业,李少林.固体脂质纳米姜黄素联合顺铂对卵巢癌SKOV3的增殖及Bax/Bcl-2蛋白表达的实验研究[J].重庆医科大学学报,2011,36(06):650-653. DOI： 10.13406/j.cnki.cyxb.2011.06.022.

DENG Shu-ting1, GAN Lin2, ZHOU Qi1, et al. Effect of curcumin-loaded solid lipid nanoparticles and DDP on proliferation and expression of Bax/Bcl-2 protein in human ovarian cell line SKOV3[J]. Journal of Chongqing Medical University, 2011,36(6):650-653. DOI： 10.13406/j.cnki.cyxb.2011.06.022.

摘要

目的:研究固体脂质纳米姜黄素（Curcumin-loaded solid lipid nanoparticles

SLN-Cur）与顺铂（Cisplatin

DDP）联用对人卵巢癌SKOV3细胞株生长的影响及促凋亡作用。方法:姜黄素（Curcumin

Cur）制备成SLN-Cur新型给药系统

分别检测DDP、Cur、SLN-Cur、DDP+Cur、DDP+SLN-Cur对人卵巢癌SKOV3细胞株凋亡形态学变化及超微结构变化

细胞凋亡率及凋亡相关基因蛋白的表达。结果:SLN-Cur可抑制SKOV3细胞生长

抑制率与药物浓度及作用时间成依赖关系

24 h IC50=40.14μmol/L

DDP与SLN-Cur联合诱导细胞凋亡作用增强

具有显著协同作用;Bcl-2表达减弱

Bax表达增强。结论:SLN-Cur合用DDP对SKOV3细胞株具有较好的抑制增殖及促凋亡作用

通过下调Bcl-2表达及上调Bax表达而诱导细胞凋亡。

Abstract

Objective:To investigate the effect of curcumin-loaded solid lipid nanoparticles(SLN-Cur)in combination with cisplatin(DDP)on growth of human ovarian cancer cell line SKOV3 and its apoptosis induction.Methods:SLN-Cur was prepared in an aqueous system using curcumin as a model drug.SKOV3 cells were treated with curcumin

DDP

SLN-Cur

curcumin combined with DDP and SLN-Cur combined with DDP respectively.Cell morphology variation and cell ultrastructure were observed;the apoptosis rate of SKOV3 cell and the expression of apoptosis-associated gene proteins were detected.Results:Curcumin-loaded solid lipid nanoparticles(SLN-Cur)could effectively inhibit SKOV3 in a dose-and-time-dependent manner.The IC50 of curcumin-loaded solid lipid nanoparticles(SLN-Cur)to SKOV3 was 40.14 μmol/L in 24 hours.Simultaneous administration of SLN-Cur and DDP resulted in synergistic cytotoxicity and apoptosis for SKOV3 cells;the expression of Bcl-2 was down-regulated while the expression of Bax was up-regulated.Conclusion:The rate of cell proliferation inhibition and the rate of the SKOV3 cell line apoptosis by SLN-Cur combined with DDP are significantly higher.The apoptosis is related to the down-regulation of Bcl-2 and up-regulation of Bax.

关键词

固体脂质纳米粒姜黄素顺铂人卵巢癌SKOV3细胞株细胞凋亡

Keywords

solid lipid nanoparticlescurcuminCisplatin(DDP)human ovarian cancer cell line SKOV3cell apoptosis

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