Objective:To investigate the effect and the mechanism of D-ribose on myocardial ischemia/reperfusion injury of rat hearts.Methods:24 SD rats were randomly divided into sham group

ischemia/reperfusion group(I/R group)and D-ribose group(n=8).The rats in sham group did not undergo the left anterior descending coronary artery(LAD)ligation;The rats in I/R group underwent the LAD ligation for 30 min

then were reperfused for 120 min;The rats in D-ribose group(D-ribose group)were administered D-ribose(800 mg/kg)through tail vein 30 min prior to the LAD ligation.The left ventricular samples of the three groups were prepared for investigation.The expression of CINC-1mRNA was detected by RT-PCR;myeloperoxidase(MPO)was detected by ELISA;nuclear factor-κB(NF-κB)was detected by Western blot

and cardiac HE sections were observed under light microscope.Results:Compared with sham group

higher expression of CINC-1 mRNA

NF-κB and MPO were detected in I/R group and D-ribose group(P<0.05)with more remarkable histopathologic changes and neutrophil infiltration.Compared with I/R group

less expressions of CINC-1 mRNA

NF-κB and MPO were detected in D-ribose group(P<0.05)with less remarkable histopathologic changes and neutrophil infiltration.Conclusion:Exogenous D-ribose might play protective effect on myocardial ischemia/reperfusion injury of rat hearts.The mechanism could be related to its decreasing the expression of NF-κB

CINC-1 mRNA and MPO as well as reducing neutrophil infiltration.