Objective:To determine the effect of quercetin on mouce fibrob-last cell line L929 proliferation and explore it(s molecular mechanism.Methods:The effect of mouce fibctrob-last cell line L929 proliferation was measured by MTT assay;the effect of quercetin on protein regulation was detected by luciferase reporter assay;the effect of quercetin on the expressions of mTOR

cyclin D1 and p-4E-BP1

key genes in the mTOR-mediated protein translation pathway

was determined by Western blotting analysis.Results:Among the high concentration groups(1

1.5

2

2.5)×10-4 mol/L at the point of 36 hours

quercetin significantly induced proliferation of mouce Compared with the control group

the RLU(relative light units)of 2.5×10-4 mol/L quercetin value fibrob-last cell line L929;the Luciferase reporter gene detection system showed as follows:increased significantly at the point of 36 hours;Quercetin increased the expression of mTOR and cyclin D1

and the phosphorylation of 4E-BP1 in mouce fibro-blast cell line L929 by Western blotting

and mTOR pathway blocking experiments.After 100 ng/ml rapamycin for 36 h

compared with the control group

the P-4E-BP1

mTOR and cyclin D1 protein expressions of quercetin group were significantly increased;those of rapamycin group were significantly reduced;and those of rapamycin + quercetin group were increased

but decreased compared with quercetin group.Conclusion:Quercetin can induce mouce fibrob-last cell line L929 proliferation through activating mTOR signal pathway to increase expression of cyclin D1.