运动锻炼对局灶性脑缺血大鼠脑皮质RGMa表达的影响

郭振委; 秦新月; 孔渝菡

doi:10.13406/j.cnki.cyxb.2011.05.034

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运动锻炼对局灶性脑缺血大鼠脑皮质RGMa表达的影响

基础研究 | 更新时间：2024-01-17

- 运动锻炼对局灶性脑缺血大鼠脑皮质RGMa表达的影响
- Effects of treadmill exercise on the expression of RGMa in infarcted brain cortex of rats after middle cerebral artery occlusion/reperfusion
- 重庆医科大学学报 2011年36卷第5期页码：578-582
- 作者机构：
  
  重庆医科大学附属第一医院神经内科重庆市高校神经病学重点实验室
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（编号:30770762,30970987）;重庆医科大学附属第一医院基金资助项目（编号:YXJJ2009-02）
- DOI：10.13406/j.cnki.cyxb.2011.05.034
  中图分类号： R743.33
- 纸质出版日期：2011，
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[1]郭振委,秦新月,孔渝菡.运动锻炼对局灶性脑缺血大鼠脑皮质RGMa表达的影响[J].重庆医科大学学报,2011,36(05):578-582.

GUO Zhen-wei, QIN Xin-yue, KONG Yu-han. Effects of treadmill exercise on the expression of RGMa in infarcted brain cortex of rats after middle cerebral artery occlusion/reperfusion[J]. Journal of Chongqing Medical University, 2011,36(5):578-582.
[1]郭振委,秦新月,孔渝菡.运动锻炼对局灶性脑缺血大鼠脑皮质RGMa表达的影响[J].重庆医科大学学报,2011,36(05):578-582. DOI： 10.13406/j.cnki.cyxb.2011.05.034.

GUO Zhen-wei, QIN Xin-yue, KONG Yu-han. Effects of treadmill exercise on the expression of RGMa in infarcted brain cortex of rats after middle cerebral artery occlusion/reperfusion[J]. Journal of Chongqing Medical University, 2011,36(5):578-582. DOI： 10.13406/j.cnki.cyxb.2011.05.034.

摘要

目的:探讨运动锻炼对卒中后大鼠缺血侧脑皮质排斥性导向分子A（RepulsiveguidancemoleculeA

RGMa）表达的影响。方法:选用SD大鼠120只

随机分为5组

正常组

假手术组

MCAo模型7、14、28 d组

以上各组再分为:非运动、低运动量、中动量、高动量锻炼组

每组6只。应用线栓法制备局灶性脑缺血/再灌注模型

用实时定量PCR和免疫组化方法检测缺血侧脑皮质RGMa的表达。结果:①实时定量PCR:低运动量模型各组与对应时间点非运动模型组比

RGMa相对表达量差异无统计学意义（P>0.05）。中运动量模型各组与各对应时间点非运动模型组相比

前者表达量显著下降（P<0.05）

高运动量模型各组与对应时间点非运动模型组相比

RGMa相对表达量显著升高（P<0.05）。②免疫组化:低运动量模型各组与对应时间点各非运动模型组相比

RGMa表达差异无统计学意义（P>0.05）。中运动量模型各组与对应时间点各非运动模型组相比

前者RGMa表达明显下降（P<0.05）

但中运动量模型7 d组RGMa表达量与对应非运动模型组相比差异无统计学意义（P>0.05）

高运动量模型各组与对应时间点各非运动模型组相比

RGMa表达明显增加（P<0.05）。神经功能评分显示中运动量模型组明显改善神经功能（P<0.05）。结论:适量锻炼可以降低局灶性脑缺血大鼠患侧皮质RGMa的表达

改善神经功能。

Abstract

Objective:To observe the expression of RGMa in infarcted brain cortex of rats with middle cerebral artery occlusion(MCAo)after experiencing treadmill exercise.Methods:120 Sprague-Dawley rats were randomly divided into 5 groups:control group

sham-operation group

and 7

28 d after ischemia group.Then each group above were randomly divided into four groups:sedentary group

low-intensity

moderate-intensity

high-intensity treadmill exercise group with MCAo.The MCAo/reperfusion model was induced by ligation with nylon monofilament in rats in the study.Results:①Real-time quantitative PCR(RT-qPCR):There are no significant differences between each low-intensity treadmill MCAo group and each sedentary MCAo group for expression of RGMa mRNA(P>0.05).There was down-regulation of it in each moderate-intensity treadmill exercise MCAo group

compared with each sedentary MCAo group(P<0.05).But there was up-regulation in high-intensity treadmill exercise MCAo group(P<0.05).②The result of immunohistochemistry:There was no significant difference between each sedentary MCAo group and each low-intensity treadmill exercise MCAo group(P>0.05).The expression of RGMa was down-regulated in moderate-intensity treadmill exercise MCAo group

except at 7 d

compared with each sedentary MCAo group(P<0.05).But the opposite result was got in high-intensity treadmill exercise MCAo group(P<0.05).Neurological deficit scores test showed moderate-intensity treadmill exercise improved neurological function in MCAo group(P<0.05).Conclusion:Moderate-intensity treadmill exercise decreased the expression of RGMa in the peri-ischemia cortex after ischemia stroke and the impaired neural function was improved.

关键词

运动锻炼脑缺血排斥性导向分子A实时定量PCR

Keywords

treadmill exercisecerebral ischemiaRGMaRT-qPCR

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