宫颈癌HeLa细胞抑癌蛋白FBW7调控机制研究

孙迪; 沈宜; 谢莹珊; 隆霜

doi:10.13406/j.cnki.cyxb.2011.05.025

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宫颈癌HeLa细胞抑癌蛋白FBW7调控机制研究

恶性肿瘤的诊断与治疗 | 更新时间：2024-01-17

- 宫颈癌HeLa细胞抑癌蛋白FBW7调控机制研究
- Study on the regulatory mechanism of tumor suppressor protein FBW7 in cervical cancer HeLa cells
- 重庆医科大学学报 2011年36卷第5期页码：520-523
- 作者机构：
  
  重庆医科大学基础医学院干细胞与组织工程研究室
- 作者简介：
- 基金信息：
  
  重庆市渝中区科委资助项目（编号:2009）
- DOI：10.13406/j.cnki.cyxb.2011.05.025
  中图分类号： R737.33
- 纸质出版日期：2011，
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[1]孙迪,沈宜,谢莹珊,隆霜.宫颈癌HeLa细胞抑癌蛋白FBW7调控机制研究[J].重庆医科大学学报,2011,36(05):520-523.

SUN Di, SHEN Yi, XIE Ying-shan, et al. Study on the regulatory mechanism of tumor suppressor protein FBW7 in cervical cancer HeLa cells[J]. Journal of Chongqing Medical University, 2011,36(5):520-523.
[1]孙迪,沈宜,谢莹珊,隆霜.宫颈癌HeLa细胞抑癌蛋白FBW7调控机制研究[J].重庆医科大学学报,2011,36(05):520-523. DOI： 10.13406/j.cnki.cyxb.2011.05.025.

SUN Di, SHEN Yi, XIE Ying-shan, et al. Study on the regulatory mechanism of tumor suppressor protein FBW7 in cervical cancer HeLa cells[J]. Journal of Chongqing Medical University, 2011,36(5):520-523. DOI： 10.13406/j.cnki.cyxb.2011.05.025.

摘要

目的:观察MAPK信号通路阻断剂UO-126对人类宫颈癌细胞株HeLa细胞周期素E（cyclinE）及F-盒和含蛋白7的WD重复结构域FBW7表达的影响。方法:采用RT-PCR、Western blot检测UO-126阻断MAPK信号通路前后cyclin E和FBW7 mRNA及蛋白表达情况。结果:RT-PCR及Western blot结果显示

UO-126作用后HeLa细胞cyclin E mRNA表达水平无明显变化

cyclin E蛋白表达水平明显下降

FBW7 mRNA及蛋白表达水平较未处理HeLa细胞均明显增高。结论:宫颈癌HeLa细胞中FBW7异常表达与MAPK信号通路激活密切相关。UO-126阻断MAPK信号通路可下调cyclin E表达

上调FBW7表达。

Abstract

Objective:To investigate the effects of UO-126

a MAPK inhibitor

on the expression of cyclin E and F-box and WD repeat domain-containing protein 7(FBW7)of human cervical cancer cell line(HeLa cells).Methods:The mRNA and protein expression of cyclin E and FBW7 were detected by RT-PCR and Western blot before and after MAPK signal was blocked by UO-126

a MAPK inhibitor.Results:RT-PCR and Western blot exhibited that cyclin E mRNA expression had no significant changes and cyclin E protein had significantly decreased after HeLa cells were treated with UO-126

and FBW7 mRNA and protein expression had significantly increased after HeLa cells were treated with UO-126.Conclusion:The abnormal expression of FBW7 is closely related to the activation of MAPK signaling pathway in cervical cancer HeLa cells.Cyclin E is decreased and FBW7 is increased after UO-126 blocks the MAPK signaling pathway.

关键词

UO-126MAPK宫颈癌细胞株（HeLa细胞）细胞周期素EFBW7

Keywords

UO-126MAPKcervical cancer HeLa cellscyclin EFBW7

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