Objective:To explore the expression of Tribble3(TRB3)in diabetic cardiomyopathy

and the relationship between TRB3 and diabetic cardiomyopathy.Methods:24 SD male rats were divided into control group(n=12)and diabetes group(n=12)randomly.Diabetes was induced by a single peritoneal injection of strepotozotocin(STZ

60 mg/kg).8 weeks later

blood sugar and HbAc1 were tested.The changes of myocardium organization structure were observed by HE.Interstitial fibrosis was detected by Masson.Apoptosis of cardiomyocyte determined by Tunel.TRB3 of each group was tested by quantitative real time-PCR and Western blot separately.Results:Compared with control group

there were such changes in diabetes group as the disorder and fracture of cardiocytes

and infiltration of inflammatory cells.There were more myocardial collagen content(P<0.01)and much more apoptosis of cardiomyocyte(P<0.01)in the hearts of diabetic rats.The expression of TRB3 in protein and mRNA was up-regulated(P<0.01).Significant positive correlations were observed between TRB3 protein and apoptosis(r=0.668

P<0.05)

and between TRB3 protein and interstitial fibrosis(r=0.595

P<0.05).Conclusion:There are obviously increasing of protein and mRNA of TRB3 in diabetic cardiomyopathy.TRB3 may promote the development of diabetic cardiomyopathy by inducing apoptosis of cardiac myocyte and enhancing interstitial fibrosis.