Objective:To study the treatment of high intensity focused ultrasound(HIFU) in synaptophysin(SYP)of neonatal rats with hypoxic-ischemic brain damage(HIBD).Methods:One hundred and ninety-two 7-day-old SD rats were randomly divided into four groups:1 sham group(n=48

A)

2 HIBD model group(n=48

B)

3 acupuncture group(n=48

C)

4 high intensity focused ultrasound group(n=48

D)

every group respectively divided into 1w

2w

3w sub-groups(n=8).The expressions of synaptophysin(SYP)in Hippocampus were examined with immunohistochemical staining and RT-PCR.Results:In high intensity focused ultrasound group and acupuncture group

SYP expression levels were significantly higher than those in sham group and HIBD model group(P<0.05)

which had distinct differences compared with the HIBD model group.The expressions of SYP in high intensity focused ultrasound group had no difference compared with acupuncture group.Conclusion:High-intensity focused ultrasound therapy can enhance the expression of SYP level

which has neuroprotective function.Its possible mechanism is that when HIFU is focused on the point

it can play a similar role like acupuncture which can increase the synaptic of HIBD.