Objective:To construct the eukaryotie expression vector of proliferin related protein

and to investigate its biological function.Methods:The coding region of proliferin related protein amplified by RT-PCR from mouse testis RNA was cloned into the pEGFP-C1 vector to form the recombinant PRP-pEGFP-C1 plasmid.Then the recombinant plasmid was transfected into 293FT cells

and the expression of PRP was detected by RT-PCR and Western blotting.MTT and flow cytometry(FCM)analysis were employed to analysis the cell proliferation activity and the cell cycle

respectively.Results:PRP coding region was successfully cloned into the eukaryotic vector pEGFP-C1.The recombinant vector was transfected into 293FT

results of RT-PCR and Western blotting showed that the PRP could express in 293FT cells.Overexpressing PRP gene decreased the cell proliferation activity as well as disturbing the cell cycle distribution of 293FT cells in vitro.Conclusion:We have constructed a recombinant vector expressing PRP and eGFP fusion protein

which is in favour of the further study on the gene function of PRP.In addition

PRP has anti-proliferin activity in human-derived cells through disturbing the cell cycle

suggesting that PRP maybe act as a potential gene therapeutic candidate used in human tumor

not just mouse tumor.