Objective:To establish an HPLC method for the determination of vinorelbine tartrate in and out of cells.Methods:The vinorelbine tartrate was frozen and defrosted time and again.Then the sample was extracted with methanol and determined through Lichrospher C18(4.6 mm×250 mm

5 μm)column in a mobile phase of 50 mmol/ml potassium phosphate buffer(pH 3.5) and acetonitrile(70:30 v/v) with the flow rate of 1.0 ml/min at 30℃.The detection wavelength was 269 nm.Result: It was accurate and sensitive with a linear range from 0.5 μg/ml to 25 μg/ml for Vinorelbine Tartrate.The mean extraction recovery of extracellular fluid obtained was 84.6%

and the absolute recovery was 100.3%.Intra-and inter-day periods expressed as percent R.S.D.were≤4.84%.For intracellular fluid

the mean extraction recovery was 88.2% and the absolute recovery was 98.3%.Intra-and inter-day periods expressed as percent R S D were≤4.69%.The lowest limit of quantification was 0.5 μg/ml.Conclusion:This method is simple

rapid

accurate and selective for the determination of VCR.