辛伐他汀诱导K562细胞凋亡过程中细胞内钙离子浓度、Calbindin-D28K和calpain基因的变化

曾娅莉; 黄文芳; 刘华; 杨永长; 邓建军; 刘俭

doi:10.13406/j.cnki.cyxb.2011.03.040

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辛伐他汀诱导K562细胞凋亡过程中细胞内钙离子浓度、Calbindin-D28K和calpain基因的变化

基础研究 | 更新时间：2024-01-17

- 辛伐他汀诱导K562细胞凋亡过程中细胞内钙离子浓度、Calbindin-D28K和calpain基因的变化
- Changes of expression levels of Ca²⁺,D28K（Calbindin-D28K） and calpain in K562 cell in the process of apoptosis induced by simvastatin
- 重庆医科大学学报 2011年36卷第3期页码：277-280
- 作者机构：
  
  1. 四川绵阳四○四医院
  2. 川北医学院附属第二医院检验科
  3. 四川省医学科学院四川省人民医院检验科
- 作者简介：
- 基金信息：
  
  四川省卫生厅科研基金资助项目（编号:060119）
- DOI：10.13406/j.cnki.cyxb.2011.03.040
  中图分类号： R96
- 纸质出版日期：2011，
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[1]曾娅莉,黄文芳,刘华,杨永长,邓建军,刘俭.辛伐他汀诱导K562细胞凋亡过程中细胞内钙离子浓度、Calbindin-D28K和calpain基因的变化[J].重庆医科大学学报,2011,36(03):277-280.

ZENG Ya-li, et al. Changes of expression levels of Ca²⁺,D28K（Calbindin-D28K） and calpain in K562 cell in the process of apoptosis induced by simvastatin[J]. Journal of Chongqing Medical University, 2011,36(3):277-280.
[1]曾娅莉,黄文芳,刘华,杨永长,邓建军,刘俭.辛伐他汀诱导K562细胞凋亡过程中细胞内钙离子浓度、Calbindin-D28K和calpain基因的变化[J].重庆医科大学学报,2011,36(03):277-280. DOI： 10.13406/j.cnki.cyxb.2011.03.040.

ZENG Ya-li, et al. Changes of expression levels of Ca²⁺,D28K（Calbindin-D28K） and calpain in K562 cell in the process of apoptosis induced by simvastatin[J]. Journal of Chongqing Medical University, 2011,36(3):277-280. DOI： 10.13406/j.cnki.cyxb.2011.03.040.

摘要

目的:观察辛伐他汀诱导K562细胞凋亡时细胞内Ca2+浓度、Ca2+调节蛋白（Calbindin-D28K

D28K）和Ca2+激活蛋白（calpain）基因变化

以探讨K562细胞凋亡机制。方法:常规培养细胞24 h

20μmol/L辛伐他汀处理K562细胞48 h;流式细胞技术检测细胞凋亡率和Ca2+浓度

PCR技术检测D28K和calpain基因。结果:20μmol/L辛伐他汀作用K562细胞24、48、72 h凋亡率改变分别为（6.10±0.35）%、（14.15±0.42）%、（30.70±0.65）%

随药物作用时间延长细胞凋亡率增加;辛伐他汀作用K562细胞12、24、48、72 h

游离钙荧光强度变化为52.93±2.85、19.63±1.56、13.81±1.05、7.84±0.98

各处理组荧光强度与对照组相比升高（P<0.05）;与相同时间对照组比较

calpain和D28K基因分别在辛伐他汀作用K562细胞24 h和48 h后上调（P<0.05）。结论:辛伐他汀诱导K562细胞凋亡时细胞内Ca2+浓度显著升高

细胞内Ca2+浓度调节蛋白D28K和calpain基因上调

这些改变可能是辛伐他汀诱导K562细胞凋亡的机制之一。

Abstract

Objective: To observe the changes of Ca2+ level and calbindin-D28K and calpain gene

and investigate the mechanism in the process of simvastatin-induced apoptosis of K562 cells.Methods: K562 cells cultivated 24h were exposed in different concentrations of 20 μmol/L simvastatin.Flow cytometry was performed to confirm cell apoptotic ratio and analyze Ca2+ level.The gene expression of D28K and calpain were analyzed by RT-PCR(reverse transcription-PCR).Results: K562 cells could be induced to apoptosis after 20 μmol/L simvastatin treatment for 24 h

48 h and 72 h

and the apoptotic ratios were 6.1%±0.35%

14.15%±0.42%

and 30.70%±0.65% respectively by flow cytometric assay(Annexin V/PI double staining).There was a significant increase in the drug-treated groups compared with the control groups after 24 h.The Ca2+ level was detected by Fluo-3AM and was markedly increased in treated groups compared with the control groups at different time points(P<0.05).The mRNA of D28K and calpain expression levels in drug-treated group were up-regulated.Conclusion: K562 cells could be induced to apoptosis by simvastatin.The free of Ca2+ may take part in the apoptosis in early period.The potential mechanism of the K562 cell apoptosis might be related tothe up-regulated calbindin-D28k and calpain gene.

关键词

辛伐他汀D28K细胞内钙离子钙离子激活蛋白细胞凋亡

Keywords

simvastatincalbindin-D28Kcalciumcalpainapoptosis

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