Objective: To express the capsids of the predominant strain NoV(GⅡ-4) of our country and explore its binding activity and pattern model with HBGAs receptor.Methods: GZ121 NoV ORF2 gene was constructed into baculovirus expression system.After the transfection to sf9 cells

the recombinant proteins were purified by sucrose-ultra centrifugation

and then were identified by SDS-PAGE

Western blot and TEM.And the binding patterns of NoV to HBGAs antigens were identified by EIA.Results: VLPs expression was confirmed by using SDS-PAGE

Western blot and could bind to all secretors(OD>2.5)

but not to all nonsecretors(OD≈0.3).Conclusion: The GⅡ-4 NoV VLP was successfully expressed and HBGAs receptor binding assays were established.These data will help us to explore the host adaptation character of NoV and apply the assays to screen drugs for antivirals against Noroviruses.The results obtained in the present work will also lay a foundation for the development of the immunological diagnostic reagents as well as vaccines for Norovirus.