双特异抗体增效骨髓间充质干细胞干预心肌纤维化

邓玮; 陈庆伟; 李兴升; 刘灏; 牛司强; 周玥; 李桂琼; 柯大智; 莫显刚

doi:10.13406/j.cnki.cyxb.2011.02.015

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双特异抗体增效骨髓间充质干细胞干预心肌纤维化

基础研究 | 更新时间：2024-01-17

- 双特异抗体增效骨髓间充质干细胞干预心肌纤维化
- Effect of bone marrow mesenchymal stem cells transplantation on fibroid myocardium with the aid of bispecific antibody
- 重庆医科大学学报 2011年36卷第2期页码：129-132
- 作者机构：
  
  1. 重庆医科大学附属第二医院老年病科
  2. 重庆医科大学附属第二医院消化内科
  3. 重庆医科大学检验系
  4. 重庆医科大学基础医学院组织学与胚胎学教研室
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（编号:30970826）
- DOI：10.13406/j.cnki.cyxb.2011.02.015
  中图分类号： R541
- 纸质出版日期：2011，
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[1]邓玮,陈庆伟,李兴升,刘灏,牛司强,周玥,李桂琼,柯大智,莫显刚.双特异抗体增效骨髓间充质干细胞干预心肌纤维化[J].重庆医科大学学报,2011,36(02):129-132.

DENG Wei, et al. Effect of bone marrow mesenchymal stem cells transplantation on fibroid myocardium with the aid of bispecific antibody[J]. Journal of Chongqing Medical University, 2011,36(2):129-132.
[1]邓玮,陈庆伟,李兴升,刘灏,牛司强,周玥,李桂琼,柯大智,莫显刚.双特异抗体增效骨髓间充质干细胞干预心肌纤维化[J].重庆医科大学学报,2011,36(02):129-132. DOI： 10.13406/j.cnki.cyxb.2011.02.015.

DENG Wei, et al. Effect of bone marrow mesenchymal stem cells transplantation on fibroid myocardium with the aid of bispecific antibody[J]. Journal of Chongqing Medical University, 2011,36(2):129-132. DOI： 10.13406/j.cnki.cyxb.2011.02.015.

摘要

目的:探讨在双特异抗体（Bispecificantibody

BiAb）的辅助下

骨髓间充质干细胞（Bonemarrowmesenchymalstemcells

BMSCs）移植干预心肌纤维化的效果。方法:以anti-CD29（能识别间充质干细胞）和抗肌凝蛋白轻链抗体（Anti-myosin light chainantibody

AMLCA）（能特异性结合损伤心肌）制备BiAb（CD29×AMLCA）。将此BiAb与雄性小鼠BMSCs经尾静脉输入异丙肾性心肌纤维化雌性小鼠（BMSCs+BiAb组）。另设单纯BMSCs组、单纯BiAb组、正常对照组、未治疗组。5周后处死小鼠

荧光定量PCR检测心肌y染色体鉴别基因（Sex-determiningregion ofY-chromosome

SRY）、基质金属蛋白酶-9（Matrixmetalloproteinas-es-9

MMP-9）、基质金属蛋白酶组织抑制剂-1（Tissue inhibitor of metalloproteinase-1

TIMP-1）的表达。天狼猩红染色比较心脏胶原含量。结果:BMSCs+BiAb组的干细胞归巢数比BMSCs组多（P<0.05）。与未治疗组相比

BMSCs+BiAb组和单纯BMCSs组的心肌MMP-9和TIMP-1表达下调

心脏胶原沉积降低（P<0.05）。与BMSCs组相比

BMSCs+BiAb组的心肌纤维化改善更为明显（P<0.05）。结论:CD29×AMLCA双特异抗体可促进小鼠骨髓间充质干细胞的归巢。BMSCs归巢后能改善缺血心肌MMP-TIMP表达

干预心肌纤维化。

Abstract

Objective: To investigate the effect of bone marrow mesenchymal stem cells(BMSCs) transplantation on fibroid myocardium with the aid of bispecific antibody(BiAb).Methods: BiAb [anti-CD29×anti-myosin light chain antibody(AMLCA)] was prepared and combined with isolated BMSCs from male mice and transfused into female mice with isoproterenol-induced myocardial fibrosis via tail vein.This study included five groups: BMSCs+BiAb

BMSCs

BiAb

untreated

and control groups.Five weeks after treatment

expression levels of sex-determining region of Y-chromosome(SRY)

matrix metalloproteinases-9(MMP-9)

tissue inhibitor of metalloproteinase-1(TIMP-1) in myocardium were detected by fluorescent qRT-PCR.Collagen distribution was observed using sirius red staining.Results: Higher homing number of BMSCs was shown in the BMSCs+BiAb group than that of the BMSCs group(P<0.05).Compared with the untreated group

BMSCs+BiAb and BMSCs groups had decreased levels of MMP-9

TIMP-1 and collagen deposition(P<0.05).Decreased level of collagen content was significantly different in BMSCs+BiAb as compared to BMSCs group(P<0.05).Conclusion: Homing rate and repairing efficacy of BMSCs improve via treatment with combination of BiAb.BMSCs can improve MMP-TIMP expression in injured myocardium and interfere with myocardial fibrosis after homing.

关键词

骨髓间充质干细胞双特异性抗体心肌纤维化细胞移植

Keywords

bone marrow mesenchymal stem cellbispecific antibodymyocardial fibrosiscell transplantation

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