CDH12基因siRNA对涎腺腺样囊性癌细胞侵袭力的影响

苏柏华; 王锦锋; 佘林; 郑斐斐; 丁林灿; 卢友光

doi:10.13406/j.cnki.cyxb.2011.01.035

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CDH12基因siRNA对涎腺腺样囊性癌细胞侵袭力的影响

基础研究 | 更新时间：2024-01-17

- CDH12基因siRNA对涎腺腺样囊性癌细胞侵袭力的影响
- Role of CDH12 siRNA on invasiveness of salivary adenoid cystic carcinoma cells
- 重庆医科大学学报 2011年36卷第1期页码：11-15
- 作者机构：
  
  福建医科大学附属口腔医院预防科
- 作者简介：
- 基金信息：
  
  福建省科技厅科技计划重点项目（编号:2008Y0041）
- DOI：10.13406/j.cnki.cyxb.2011.01.035
  中图分类号： R739.870.22
- 纸质出版日期：2011，
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[1]苏柏华,王锦锋,佘林,郑斐斐,丁林灿,卢友光.CDH12基因siRNA对涎腺腺样囊性癌细胞侵袭力的影响[J].重庆医科大学学报,2011,36(01):11-15.

SU Bo-hua, et al. Role of CDH12 siRNA on invasiveness of salivary adenoid cystic carcinoma cells[J]. Journal of Chongqing Medical University, 2011,36(1):11-15.
[1]苏柏华,王锦锋,佘林,郑斐斐,丁林灿,卢友光.CDH12基因siRNA对涎腺腺样囊性癌细胞侵袭力的影响[J].重庆医科大学学报,2011,36(01):11-15. DOI： 10.13406/j.cnki.cyxb.2011.01.035.

SU Bo-hua, et al. Role of CDH12 siRNA on invasiveness of salivary adenoid cystic carcinoma cells[J]. Journal of Chongqing Medical University, 2011,36(1):11-15. DOI： 10.13406/j.cnki.cyxb.2011.01.035.

摘要

目的:探讨钙粘素12（Cadherin12

CDH12）对涎腺腺样囊性癌（Salivary adenoid cystic carcinoma

SACC）细胞侵袭和转移能力的影响。方法:以人涎腺腺样囊性癌高转移细胞株（Salivary adenoid cystic carcinoma cell line with high metastatic ability

SACC-M）为研究对象

利用CDH12小分子干扰RNA（Small interfering RNAs

iRNA）对CDH12基因进行沉默

Western blot检测转染前后CDH12的表达变化

Cell Counting Kit-8法检测转染前后细胞的生长速度

体外侵袭实验比较细胞侵袭能力变化

体外迁移运动实验比较细胞运动能力的改变。结果:CDH12 siRNA明显下调CDH12基因的表达。CDH12表达下调后

SACC-M细胞体外侵袭能力显著降低

体外迁移运动能力明显降低。结论:CDH12明显地促进了SACC细胞的体外侵袭和迁移运动

提示CDH12可能在SACC的恶性进展中起着重要作用。

Abstract

Objective:To study the effect of CDH12 on invasion and migration of human salivary adenoid cystic carcinoma cells in vitro.Methods:The highly metastatic human salivary adenoid cystic carcinoma cell line SACC-M was transfected by CDH12 siRNA to silence CDH12 gene.The protein expression of CDH12

before and after siRNA transfection

was examined by Western blot.Cell growth rate was determined with Cell Counting Kit-8.At the same time

the invasive capability and migration capability were evaluated by tumour invasion assay and migration assay

respectively.Results CDH12 siRNA transfection specifically down-regulated the CDH12 protein in SACC-M cells.The downregulation of CDH12 supressed the invasive capability and migration capability of SACC-M cells.Conclusion:CDH12 obviously promotes the invasion and migration of SACC cells in vitro.These results suggest that CDH12 may play an important role in the malignant progression in salivary adenoid cystic carcinoma.

关键词

小分子干扰RNA钙粘素12腺样囊性癌侵袭

Keywords

siRNACDH12adenoid cystic carcinomainvasion

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