葡萄糖调节蛋白78与胃癌细胞化疗敏感性的关系

李禄椿; 高青; 颜晓丽

doi:10.13406/j.cnki.cyxb.2011.01.027

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葡萄糖调节蛋白78与胃癌细胞化疗敏感性的关系

基础研究 | 更新时间：2024-01-17

- 葡萄糖调节蛋白78与胃癌细胞化疗敏感性的关系
- Research on the relation between expression GRP78 in gastric cancer cell lines and its sensitivity to chemotherapeutic drugs
- 重庆医科大学学报 2011年36卷第1期页码：46-50
- 作者机构：
  
  重庆医科大学附属第一医院消化内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.01.027
  中图分类号： R735.2
- 纸质出版日期：2011，
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[1]李禄椿,高青,颜晓丽.葡萄糖调节蛋白78与胃癌细胞化疗敏感性的关系[J].重庆医科大学学报,2011,36(01):46-50.

LI Lu-chun, et al. Research on the relation between expression GRP78 in gastric cancer cell lines and its sensitivity to chemotherapeutic drugs[J]. Journal of Chongqing Medical University, 2011,36(1):46-50.
[1]李禄椿,高青,颜晓丽.葡萄糖调节蛋白78与胃癌细胞化疗敏感性的关系[J].重庆医科大学学报,2011,36(01):46-50. DOI： 10.13406/j.cnki.cyxb.2011.01.027.

LI Lu-chun, et al. Research on the relation between expression GRP78 in gastric cancer cell lines and its sensitivity to chemotherapeutic drugs[J]. Journal of Chongqing Medical University, 2011,36(1):46-50. DOI： 10.13406/j.cnki.cyxb.2011.01.027.

摘要

目的:探讨葡萄糖调节蛋白78（Glucose regulated protein78

GRP78）在不同胃癌细胞株中的表达和对化疗药物敏感性的关系

并探讨其初步机制。方法:用RT-PCR及Western blot法检测分化程度不同的胃癌细胞株MKN45、SGC7901和NCI-N87中GRP78的表达

并分别观察经Ca2+载体A23187和Ca2+螯合剂BAPTA-AM处理后的SGC7901中GRP78的表达

MTT法检测各组胃癌细胞株对多种化疗药物的敏感性。Western blot法检测SGC7901及分别经A23187和BAPTA-AM处理后细胞AKT/PKB蛋白表达水平

流式细胞仪检测细胞的凋亡率。结果:GRP78在3种胃癌细胞系中均有表达

在MKN45中为高表达

对化疗药物的敏感性最低;在NCI-N87中为低表达

但对化疗药物的敏感性最高。A23187组SGC7901细胞GRP78mRNA和蛋白及AKT/PKB蛋白的表达显著上调

对长春新碱（Vincristine

VCR）的敏感性降低;而在BAPTA-AM组则与之相反。结论:GRP78在不同分化的胃癌细胞株中表达水平不同

对化疗药物的敏感性亦不同。改变胃癌细胞GRP78的表达能影响其下游蛋白AKT/PKB的表达水平

从而影响胃癌细胞对化疗药物的敏感性。

Abstract

Objective:To research the sensitivity of different gastric cancer cell lines to chemotherapeutic drugs and evaluate GRP78 expression in order to analyze the relation between them.Methods:The SGC7901 cells were treated by calcium ionophore A23187 and Ca2+ chelator BAPTA-AM.The sensitivity of different gastric cancer cell lines to different chemotherapeutic drugs was detected by MTT assay.The expression of GRP78 was assessed by RT-PCR and Western blot.The expression of AKT/PKB in SGC7901

A23187-SGC7901 and BAPTA-AM-SGC7901 was analyzed by Western blot.Cell apoptosis was analyzed by flow cytometry in order to evaluate the therapeutic sensitivity to VCR.Results:GRP78 was expressed in all of the three gastric cancer cell lines.The expression of GRP78 at both protein and mRNA levels was the lowest in NCI-N87 cell

whose sensitivity to different chemotherapeutic drugs was the highest one.The expression of GRP78 at both protein and mRNA levels was the highest in MKN45 cell

whose sensitivity to different chemotherapeutic drugs was the lowest one.GRP78 was up-regulated and down-regulated by A23187 and BAPTA-AM respectively in gastric cancer cell line SGC7901.The sensitivity to VCR was the highest in BAPTA-AM-SGC7901

whose expression of AKT/PKB was the lowest one.On the contrary

the sensitivity to VCR was the lowest in A23187-SGC7901 whose expression of AKT/PKB was the highest.Conclusion:The expression of GRP78 in three gastric cancer cells is diverse

and the sensitivity of those cells to chemotherapeutic drugs is also different.Altering expression of GRP78 in gastric cancer cell can affect its downstream protein AKT/PKB expression levels

thereby affecting the sensitivity of gastric cancer cells to chemo-therapeutic drugs.

关键词

胃癌葡萄糖调节蛋白78敏感性

Keywords

gastric cancerglucose-regulated protein78sensitivity

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