WANG Yan-li, et al. Effects of PI3K/Akt inhibition on the metastasis and invasion of human gastric carcinoma cell SGC-7901 and its mechanism[J]. Journal of Chongqing Medical University, 2011,36(1):27-30.
WANG Yan-li, et al. Effects of PI3K/Akt inhibition on the metastasis and invasion of human gastric carcinoma cell SGC-7901 and its mechanism[J]. Journal of Chongqing Medical University, 2011,36(1):27-30. DOI: 10.13406/j.cnki.cyxb.2011.01.026.
Objective:To investigate the influence of PI3K/Akt on metastasis and invasion of human gastric carcinoma.Methods:Human gastric carcinoma cell line SGC-7901 was cultured with PI3K/Akt signaling pathway inhibitor LY294002 in vitro.The expression of total Akt and p-Aktser473 were detected in protein level by Western blot
the cell-ground substance adhesion was tested by cell adherence assay and the invasion ability was measured with Transwell plate
the expression of E-cadherin in mRNA level and protein level were detected by RT-PCR and S-P immunohistochemistry.Results:The expression of total Akt was constant
but the protein level of p-Aktser473 was down regulated after treatment with LY294002
the adhesion rate and the invasion ability were decreased
the expression of E-cadherin in mRNA and protein were positively regulated.Conclusion:The metastasis and invasion capacity of human gastric carcinoma cell could be decreased by inhibiting PI3K/Akt
perhaps the mechanism is associated with increasing of E-cadherin expression.