电刺激对局灶性脑梗死大鼠LINGO1和NgR1表达的影响

王进平; 李光勤; 马冉冉; 米秀娟; 黄浩然; 李莉

doi:10.13406/j.cnki.cyxb.2011.01.023

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电刺激对局灶性脑梗死大鼠LINGO1和NgR1表达的影响

基础研究 | 更新时间：2024-01-17

- 电刺激对局灶性脑梗死大鼠LINGO1和NgR1表达的影响
- Effect of electrical stimulation on the expressions of LINGO1 and NgR1 in the brain of rats after focal cerebral infarction
- 重庆医科大学学报 2011年36卷第1期页码：42-46
- 作者机构：
  
  1. 重庆医科大学附属第一医院神经内科
  2. 第三军医大学新桥医院疼痛科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.01.023
  中图分类号： R743.3
- 纸质出版日期：2011，
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[1]王进平,李光勤,马冉冉,米秀娟,黄浩然,李莉.电刺激对局灶性脑梗死大鼠LINGO1和NgR1表达的影响[J].重庆医科大学学报,2011,36(01):42-46.

WANG Jin-ping, et al. Effect of electrical stimulation on the expressions of LINGO1 and NgR1 in the brain of rats after focal cerebral infarction[J]. Journal of Chongqing Medical University, 2011,36(1):42-46.
[1]王进平,李光勤,马冉冉,米秀娟,黄浩然,李莉.电刺激对局灶性脑梗死大鼠LINGO1和NgR1表达的影响[J].重庆医科大学学报,2011,36(01):42-46. DOI： 10.13406/j.cnki.cyxb.2011.01.023.

WANG Jin-ping, et al. Effect of electrical stimulation on the expressions of LINGO1 and NgR1 in the brain of rats after focal cerebral infarction[J]. Journal of Chongqing Medical University, 2011,36(1):42-46. DOI： 10.13406/j.cnki.cyxb.2011.01.023.

摘要

目的:观察电刺激对局灶性脑梗死大鼠缺血侧皮质含亮氨酸重复序列和免疫球蛋白结构域的Nogo受体作用蛋白（LRR and Ig domain-containing

Nogo Receptor-interacting protein

LINGO1）和Nogo-66受体（Nogo-66 receptor

NgR1）表达的影响

探讨电刺激促进卒中后运动功能恢复的机制。方法:利用线栓法制作SD大鼠大脑中动脉闭塞模型

将大鼠随机分为正常组、假手术组、缺血组和电刺激组。电刺激在造模90 min后进行

30 min/d。于缺血1、3、7、14、21 d利用RT-PCR和免疫组化检测缺血皮质LINGO1和NgR1的mRNA及蛋白表达。结果:电刺激组运动功能恢复较缺血组明显改善（P<0.05）。LINGO1 mRNA及蛋白表达在缺血后1 d达峰

21 d时仍然较假手术组高（P<0.01）。电刺激组7 d其LINGO1蛋白表达较缺血组低（P<0.05）

21d时LINGO1 mRNA及蛋白表达较对照组高（P<0.01）。NgR1 mRNA及蛋白表达在缺血后1 d开始上升（P<0.01）

3 d达峰（P<0.01）

缺血14 d时较假手术组无明显差异（P>0.05）。电刺激组3 d和7 d NgR1 mRNA及蛋白表达较缺血组低（P<0.05）。结论:局灶性脑梗死大鼠缺血皮质LINGO1和NgR1的表达明显上调

脑梗死急性期予以电刺激后LINGO1蛋白和NgR1 mRNA及蛋白表达下调

可能是其促进运动功能恢复的机制之一。

Abstract

Objective:To explore the mechanism of electrical stimulation improves motor recovery in a rat stroke model.Methods:Spragne-dawley(SD)rats were subjected to establish the stroke model and randomly divieded into ischemic group

electrical stimulation group

sham operation group and normal group.Electrical stimulation was used 90 min after the operation

30 min/d.The expressions of LINGO1 and NgR1 were measured with RT-PCR and immunohistochemistry at 1 d

3 d

7 d

14 d

21 d respectively after the ischemia.Results:Motor function significantly recovered in electrical stimulation group compared with the ischemic group(P<0.05).The expressions of LINGO1 mRNA and protein in ischemic group reached the peak at 1d and were still higher than sham operation group at 21 d(P<0.01).The expression of LINGO1 protein in electrical stimulation group was lower than ischemic group at 7 d(P<0.05) but the expressions of LINGO1 mRNA and protein were higher in electrical stimulation group than in ischemic group at 21d(P<0.01).The expressions of NgR1 mRNA and protein in ischemic group elevated at 1d(P<0.01)

reached the peak at 3 d(P<0.01) and returned to normal at 14 d(P>0.05).The expressions of NgR1 mRNA and protein in electrical stimulation group were lower than ischemic group at 3d and 7 d(P<0.05).Conclusions:The expressions of LINGO1 and NgR1 in the ischemic cortex increased significantly.The decreased expressions of LINGO1 protein

NgR1 mRNA and NgR1 protein induced by electrical stimulation might be one of the mechanisms of motor recovery after acute cerebral infarction.

关键词

脑梗死LINGO1NgR1电刺激

Keywords

cerebral infarctionLINGO1NgR1electrical stimulation

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