高脂诱导的NAFLD大鼠模型血清IL-6、TNF-α等的变化

敬晓琴; 袁喆

doi:10.13406/j.cnki.cyxb.2011.01.014

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高脂诱导的NAFLD大鼠模型血清IL-6、TNF-α等的变化

基础研究 | 更新时间：2024-01-17

- 高脂诱导的NAFLD大鼠模型血清IL-6、TNF-α等的变化
- Changes of plasma Interleukin-6 and TNF-α in the development of nonalcoholic fatty liver rats induced by high fat diet
- 重庆医科大学学报 2011年36卷第1期页码：31-34
- 作者机构：
  
  重庆医科大学附属第一医院感染科重庆市传染病寄生虫病学重点实验室
- 作者简介：
- 基金信息：
  
  重庆市卫生局课题（编号:2003-31）
- DOI：10.13406/j.cnki.cyxb.2011.01.014
  中图分类号： R575.5
- 纸质出版日期：2011，
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[1]敬晓琴,袁喆.高脂诱导的NAFLD大鼠模型血清IL-6、TNF-α等的变化[J].重庆医科大学学报,2011,36(01):31-34.

JING Xiao-qin, et al. Changes of plasma Interleukin-6 and TNF-α in the development of nonalcoholic fatty liver rats induced by high fat diet[J]. Journal of Chongqing Medical University, 2011,36(1):31-34.
[1]敬晓琴,袁喆.高脂诱导的NAFLD大鼠模型血清IL-6、TNF-α等的变化[J].重庆医科大学学报,2011,36(01):31-34. DOI： 10.13406/j.cnki.cyxb.2011.01.014.

JING Xiao-qin, et al. Changes of plasma Interleukin-6 and TNF-α in the development of nonalcoholic fatty liver rats induced by high fat diet[J]. Journal of Chongqing Medical University, 2011,36(1):31-34. DOI： 10.13406/j.cnki.cyxb.2011.01.014.

摘要

目的:白细胞介素-6（Interleukin-6I

L-6）和肿瘤坏死因子（Tumor necrosis factor-α

TNF-α）在非酒精性脂肪肝病（Nonalcoholic fatty liver disease

NAFLD）的形成中具有重要作用。我们探讨在大鼠NAFLD发生发展过程中IL-6血清浓度的变化及与TNF-α、胰岛素抵抗（Insulin resistance

IR）的关系

从而更好地了解非酒精性脂肪肝病的发病机理。方法:48只雄性SD大鼠随机分为正常对照组和NAFLD模型组

正常对照组采用普通饲料喂养

NAFLD模型组采用高脂饲料喂养

并于第2、4、6、10周末分批处死各组大鼠6只

并检测其血清ALT、AST、TG、TC、空腹血糖（Fasting blood glucose

FBG）、空腹胰岛素（Fastinginsulin

FINS）I、L-6、TNF-α等指标

计算肝指数及胰岛素抵抗指数（Insulin resistance index

IRI）

观察肝脏病理变化。结果:模型组大鼠血清ALT、AST、TG、TC、FINSI、RII、L-6、TNF-α均较对照组升高

其中TNF-α自第4周起高于对照组（P<0.05）I

L-6第6周起高于对照组（P<0.05）I

L-6与TNF-α（r=0.931

P<0.05）I、RI（r=0.946

P<0.05）呈高度正相关。结论:脂肪因子IL-6与TNF-αI、RI密切相关

与NAFLD的形成发展有重要关联。

Abstract

Objective:IL-6 and TNF-α play critical roles in the development of nonalcoholic fatty liver disease(NAFLD).We thus investigated the changes of plasma Interleukin-6(IL-6) and TNF-α in the nonalcoholic fatty liver of rats and their correlation.This will provide new insight into the pathology of NAFLD.Methods:Forty-eight male SD rats were divided in two groups randomly.The NAFLD was induced in male SD rats by feeding the high-lipid nutrition

whereas

the control group was fed with low-fat nutrition.All rats were sacrificed at the end of 2

and 10 weeks.The levels of ALT

AST

FBG

FINS

IL-6 and TNF-α in the sera of rats were measured.The liver index and IRI were counted and the pathologic changes of liver were recorded during experiments.Results:Compared with control group

the levels of ALT

AST

FINS

IRI

TNF-α and IL-6 in the sera of NAFLD group all increased significantly(P<0.01).The levels of TNF-α in NAFLD group started to increase significantly at week 4(P<0.05)

while the levels of IL-6 began to elevate from week 6(P<0.05).From week 2 to week 10

IL-6 was highly positively related to TNF-α(r = 0.931

P<0.05) and IRI(r = 0.946

P<0.05).Conclusion:The levels of IL-6 were highly correlated with TNF-α and IRI in the sera of NAFLD mouse models

which indicates that both IL-6 and TNF-α are crucial in the development of NAFLD.

关键词

非酒精性脂肪肝脂肪因子白细胞介素-6

Keywords

nonalcoholic fatty liver diseaseadipocytokinesinterleukin-6

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