Objective:To construct and identify a recombinant adenovirus vector expressing keratinocyte growth factor(KGF) gene.Methods:KGF gene was amplified from the plasmid pIRES2-EGFP-KGF by polymerase chain reaction(PCR) and then inserted into the plasmid pShuttle-CMV to construct pShuttle-KGF.After being confirmed by restriction enzyme digestion and DNA sequencing

the DNA encoding KGF in the new structure was inserted into the vector of recombinant plasmid adenovirus and confirmed by restriction enzyme digestion.Then the human embryonic kidney cell line 293 was transfected with correctly identified pAdxsi-KGF

and the recombinant adenovirus were amplified and purified

and the virus titer was detected using 50% tissue culture infective dose(TCID50) assay.The expression of the green fluorescent protein and KGF were detected by fluorescent microscope and ELISA after the recombinant adenovirus transfected human lung cancer A549 cell line.Results:The recombinant adenovirus pAdxsi-KGF was constructed and amplified with titer of 1.6×1010 pfu/ml;the green fluorescence proteins could be observed under fluorescent microscope in A549 24 h after transfection and with a stronger degree after 48 h

and KGF expression was detected by ELISA.Conclusion:The recombinant adenovirus pAdxsi-KGF was constructed successfully

which will be a good foundation for the research on the therapy of KGF for pulmonary fibrosis.